Characterization of acid-sensing ion channels in dorsal horn neurons of rat spinal cord

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	Characterization of acid-sensing ion channels in dorsal horn neurons of rat spinal cord
	Wu, LJ ; Duan, B ; Mei, YD ; Gao, J ; Chen, JG ; Zhuo, M ; Xu, L ; Wu, MA ; Xu, TL(徐天乐)
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2004
卷号	279 期号:42 页码:43716-43724
关键词	GATED NA+ CHANNEL MUTATIONS CAUSING NEURODEGENERATION MAMMALIAN DEGENERIN MDEG ROOT GANGLION-CELLS CAENORHABDITIS-ELEGANS HIPPOCAMPAL-NEURONS ACTIVATED CURRENTS PAIN H+ EXPRESSION
ISSN号	0021-9258
通讯作者	Xu, TL (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,tlxu@ion.ac.cn
英文摘要	Acid-sensing ion channels (ASICs) are ligand-gated cation channels activated by extracellular protons. In periphery, they contribute to sensory transmission, including that of nociception and pain. Here we characterized ASIC-like currents in dorsal horn neurons of the rat spinal cord and their functional modulation in pathological conditions. Reverse transcriptase-nested PCR and Western blotting showed that three ASIC isoforms, ASIC1a, ASIC2a, and ASIC2b, are expressed at a high level in dorsal horn neurons. Electrophysiological and pharmacological properties of the proton-gated currents suggest that homomeric ASIC1a and/or heteromeric ASIC1a + 2b channels are responsible for the proton-induced currents in the majority of dorsal horn neurons. Acidification-induced action potentials in these neurons were compatible in a pH-dependent manner with the pH dependence of ASIC-like current. Furthermore, peripheral complete Freund's adjuvant-induced inflammation resulted in increased expression of both ASIC1a and ASIC2a in dorsal horn. These results support the idea that the ASICs of dorsal horn neurons participate in central sensory transmission/modulation under physiological conditions and may play important roles in inflammation-related persistent pain.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1940]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Wu, LJ,Duan, B,Mei, YD,et al. Characterization of acid-sensing ion channels in dorsal horn neurons of rat spinal cord[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2004,279(42):43716-43724.
APA	Wu, LJ.,Duan, B.,Mei, YD.,Gao, J.,Chen, JG.,...&Xu, TL.(2004).Characterization of acid-sensing ion channels in dorsal horn neurons of rat spinal cord.JOURNAL OF BIOLOGICAL CHEMISTRY,279(42),43716-43724.
MLA	Wu, LJ,et al."Characterization of acid-sensing ion channels in dorsal horn neurons of rat spinal cord".JOURNAL OF BIOLOGICAL CHEMISTRY 279.42(2004):43716-43724.