Glutamate stimulates glutamate receptor interacting protein 1 degradation by ubiquitin-proteasome system to regulate surface expression of GluR2

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	Glutamate stimulates glutamate receptor interacting protein 1 degradation by ubiquitin-proteasome system to regulate surface expression of GluR2
	Guo, L. ; Wang, Y.
刊名	NEUROSCIENCE
	2007
卷号	145 期号:1 页码:100-109
关键词	GRIP1 degradation glutamate AMPA receptor UPS GluR2 AMPA RECEPTOR SYNAPTIC PLASTICITY DIFFERENTIAL PALMITOYLATION EXCITATORY SYNAPSES TRAFFICKING BINDING PSD-95 GRIP1 ENDOCYTOSIS SUBUNIT
ISSN号	0306-4522
通讯作者	Wang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Lab Neural Signal Transduct, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,yzwang@ion.ac.cn
英文摘要	The glutamate receptor interacting protein 1 (GRIP1) is a scaffolding protein in postsynaptic density (PSD), tethering AMPA receptors to other signaling proteins. Here we report that glutamate stimulation caused a rapid reduction in protein levels of GRIP1, but not that of glutamate receptor (GluR) 1, GluR2 and protein interacting with C kinase 1 (PICK1) in rat primary cortical neuron cultures. Down-regulation of GRIP1 by glutamate was blocked by carbobenzoxyl-leucinyl-leucinyl-leucinal (MG132), a proteasome inhibitor and by expression of K48R-ubiquitin, a dominant negative form of ubiquitin. The GRIP1 reduction was inhibited by MK-801, an N-methyl-D-aspartate (NMDA) receptor antagonist, but not by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), an AMPA receptor antagonist. EGTA and 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetra acetic acid tetrakis (BAPTA), two Ca2+ chelators, but not nifedipine, an L-type Ca2+ channel blocker, prevented GRIP1 degradation. Furthermore, MG132 prevented glutamate-stimulated reduction in surface amount of GluR2, and knockdown of GRIP1 by RNAi against GRIP1 reduced surface GluR2 in neurons. Our results suggest that glutamate induces GRIP1 degradation by proteasome through an NMDA receptor-Ca2+ pathway and that GRIP1 degradation may play an important role in regulating GluR2 surface expression. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.
学科主题	Neurosciences & Neurology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1783]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Guo, L.,Wang, Y.. Glutamate stimulates glutamate receptor interacting protein 1 degradation by ubiquitin-proteasome system to regulate surface expression of GluR2[J]. NEUROSCIENCE,2007,145(1):100-109.
APA	Guo, L.,&Wang, Y..(2007).Glutamate stimulates glutamate receptor interacting protein 1 degradation by ubiquitin-proteasome system to regulate surface expression of GluR2.NEUROSCIENCE,145(1),100-109.
MLA	Guo, L.,et al."Glutamate stimulates glutamate receptor interacting protein 1 degradation by ubiquitin-proteasome system to regulate surface expression of GluR2".NEUROSCIENCE 145.1(2007):100-109.