Differential effects of thiopental and pentobarbital on spinal GABA(A) receptors

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	Differential effects of thiopental and pentobarbital on spinal GABA(A) receptors
	Yang, Chuan-Xiu ; Zhang, Xiao-Bing ; Gong, Neng ; Wang, Meng-Ya ; Xu, Tian-Le(徐天乐)
刊名	NEUROCHEMICAL RESEARCH
	2008
卷号	33 期号:10 页码:2159-2165
关键词	thiopental pentobarbital GABA(A) receptors mechanically dissociated neuron spinal cord dorsal horn GATED ION CHANNELS DORSAL-HORN NEURONS IN-VITRO ETOMIDATE ANESTHETICS MODULATION ACTIVATION MECHANISMS SUBUNIT SITES
ISSN号	0364-3190
通讯作者	Xu, TL (reprint author), Med Coll Soochow Univ, Dept Neurobiol, 199 Ren Ai Rd,Suzhou Ind Pk, Suzhou 215123, Peoples R China,tlxu@ion.ac.cn
英文摘要	General anesthetics thiopental and pentobarbital possess very similar chemical structures whereas their clinical potency is quite different. The underlying molecular mechanism is not fully understood. This study was designed to assess the differential effects of thiopental and pentobarbital on GABA(A) receptors of mechanically dissociated rat spinal dorsal horn neurons by using whole-cell patch-clamp technique. Pentobarbital, at a concentration of 30 mu M, which markedly enhanced sub-saturated GABA-induced current (I (GABA)), had no effect on thiopental-induced maximal current. Similarly, the pentobarbital-induced maximal current was also not affected by 30 mu M thiopental. Moreover, a linear summation of thiopental-induced maximal current and pentobarbital-induced sub-maximal current was observed. In addition, pentobarbital failed to further enhance I (GABA) in the presence of thiopental at a concentration with maximal modulatory effects on I (GABA), and vice versa. Our results thus suggest that thiopental and pentobarbital might exert the GABA mimetic effects independently, but share a common mechanism to produce the GABA modulatory effects.
学科主题	Biochemistry & Molecular Biology ; Neurosciences & Neurology
收录类别	SCI
资助信息	National Natural Science Foundation of China[30621062]; National Basic Research Program of China[2006CB500803]; Chinese Academy of Sciences[KSCX2-YW-R-35]
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1700]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Yang, Chuan-Xiu,Zhang, Xiao-Bing,Gong, Neng,et al. Differential effects of thiopental and pentobarbital on spinal GABA(A) receptors[J]. NEUROCHEMICAL RESEARCH,2008,33(10):2159-2165.
APA	Yang, Chuan-Xiu,Zhang, Xiao-Bing,Gong, Neng,Wang, Meng-Ya,&Xu, Tian-Le.(2008).Differential effects of thiopental and pentobarbital on spinal GABA(A) receptors.NEUROCHEMICAL RESEARCH,33(10),2159-2165.
MLA	Yang, Chuan-Xiu,et al."Differential effects of thiopental and pentobarbital on spinal GABA(A) receptors".NEUROCHEMICAL RESEARCH 33.10(2008):2159-2165.