Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition

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	Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition
	Huang, Zhi-hui ; Wang, Ying ; Su, Zhi-da ; Geng, Jian-guo ; Chen, Yi-zhang ; Yuan, Xiao-bing ; He, Cheng
刊名	JOURNAL OF CELL SCIENCE
	2011
卷号	124 期号:2 页码:186-197
关键词	Ca2+ Cofilin Migration Olfactory ensheathing cells RhoA Slit-2 ACTIN-FILAMENT TURNOVER LIM-KINASE GROWTH CONE NEURONAL MIGRATION SLINGSHOT PHOSPHATASE AXON GUIDANCE NEURITE OUTGROWTH FAMILY GTPASES SPINAL-CORD IN-VITRO
ISSN号	0021-9533
通讯作者	Yuan, XB (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China,yuanxb@ion.ac.cn ; chenghe@smmu.edu.cn
英文摘要	Olfactory ensheathing cells (OECs) migrate from the olfactory epithelium towards the olfactory bulb during development. However, the guidance mechanism for OEC migration remains a mystery. Here we show that migrating OECs expressed the receptor of the repulsive guidance factor Slit-2. A gradient of Slit-2 in front of cultured OECs first caused the collapse of the leading front, then the reversal of cell migration. These Slit-2 effects depended on the Ca2+ release from internal stores through inositol (1,4,5)-triphosphate receptor channels. Interestingly, in response to Slit-2 stimulation, collapse of the leading front required the activation of the F-actin severing protein cofilin in a Ca2+-dependent manner, whereas the subsequent reversal of the soma migration depended on the reversal of RhoA activity across the cell. Finally, the Slit-2-induced repulsion of cell migration was fully mimicked by co-application of inhibitors of F-actin polymerization and RhoA kinase. Our findings revealed Slit-2 as a repulsive guidance factor for OEC migration and an unexpected link between Ca2+ and cofilin signaling during Slit-2-triggered repulsion.
学科主题	Cell Biology
收录类别	SCI
资助信息	National Key Basic Research Program[2006CB500702, 2006CB806600, 2007CB947100]; Ministry of Science and Technology of China[2009ZX09311-001]; National Natural Science Foundation[30770657, 30625023]
语种	英语
公开日期	2012-07-13
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1557]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Huang, Zhi-hui,Wang, Ying,Su, Zhi-da,et al. Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition[J]. JOURNAL OF CELL SCIENCE,2011,124(2):186-197.
APA	Huang, Zhi-hui.,Wang, Ying.,Su, Zhi-da.,Geng, Jian-guo.,Chen, Yi-zhang.,...&He, Cheng.(2011).Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition.JOURNAL OF CELL SCIENCE,124(2),186-197.
MLA	Huang, Zhi-hui,et al."Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition".JOURNAL OF CELL SCIENCE 124.2(2011):186-197.