Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells

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	Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells
	Zhang, Xu
刊名	NEUROCHEMICAL RESEARCH
	2008
卷号	33 期号:10 页码:2028-2034
关键词	delta-Opioid receptor tag-fused receptor large dense-core vesicle subcellular distribution PLASMA-MEMBRANE GLYCINE RECEPTOR PICHIA-PASTORIS SPINAL-CORD PROTEIN EXPRESSION TRAFFICKING AGONIST RAT LOCALIZATION
ISSN号	0364-3190
通讯作者	Zhang, X (reprint author), Chinese Acad Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,xu.zhang@ion.ac.cn
英文摘要	In small dorsal root ganglion neurons, delta-opioid receptors (DORs) have been found to be mainly distributed in the cytoplasm and often associated with the membrane of large dense-core vesicles (LDCVs) that contain neuropeptides. To study the distribution of DORs under various physiological or pharmacological conditions, the receptors fused with different tags are constructed, transfected into cells or animals, and examined with microscopy. In this study, we show that DOR with different tags have distinct patterns of subcellular distribution in neuroendocrine cells, PC12 cells. Both immunostaining and vesicle fraction analysis showed that the native DORs expressed in PC12 cells were mainly associated with LDCVs. In transfected PC12 cells, DOR tagged with Myc or hemagglutinin exhibited LDCV localization. However, DOR fused with GFP at N- or C-terminus was found to be mainly localized on the cell surface, and mediated the function of DOR agonist. Therefore, the distribution of DOR fused with GFP differs from the native DORs. These results suggest that the subcellular distribution of the receptor could be better presented by the fused tag with smaller molecular size.
学科主题	Biochemistry & Molecular Biology ; Neurosciences & Neurology
收录类别	SCI
资助信息	NNSFC[30630029, 30621062, 30325024]; MOST 973[2006CB806600]; CAS[KSCX2-YW-R-31]
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1699]
专题	上海神经科学研究所_神经所(总) 上海神经科学研究所_感觉系统研究组
推荐引用方式 GB/T 7714	Zhang, Xu. Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells[J]. NEUROCHEMICAL RESEARCH,2008,33(10):2028-2034.
APA	Zhang, Xu.(2008).Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells.NEUROCHEMICAL RESEARCH,33(10),2028-2034.
MLA	Zhang, Xu."Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells".NEUROCHEMICAL RESEARCH 33.10(2008):2028-2034.