Specific functions of Drosophila amyloid precursor-like protein in the development of nervous system and nonneural tissues

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	Specific functions of Drosophila amyloid precursor-like protein in the development of nervous system and nonneural tissues
	Guo, AK
刊名	JOURNAL OF NEUROBIOLOGY
	2004
卷号	61 期号:3 页码:343-358
关键词	Drosophila APPL brain structure cytoskeleton K+ currents ARRESTED EMBRYONIC NEUROBLASTS ALZHEIMERS-DISEASE BETA-PROTEIN AXONAL-TRANSPORT TAU-PHOSPHORYLATION NEURITE OUTGROWTH MEMORY MUTANTS K+ CHANNELS NEURONS GENE
ISSN号	0022-3034
通讯作者	Guo, AK (reprint author), Chinese Acad Sci, Inst Biophys, Ctr Brain & Cognit Sci, Lab Visual Informat Proc, 15 DaTun Rd, Beijing 100101, Peoples R China,akguo@ion.ac.cn
英文摘要	Drosophila amyloid precursor-like protein (APPL) is expressed extensively in the nervous system soon after neuronal differentiation. By utilizing different transgenic flies, we studied the physiological function of two APPL protein forms, membrane-bound form (mAPPL) and secreted form (sAPPL), in neural development. We found that neither deletion nor overexpression of APPL protein altered the gross structure of mushroom bodies in the adult brain. No changes were detected in cell types and their relative ration in embryo-derived cultures from all APPL mutants. However, the neurite length was significantly increased in mutants overexpressing mAPPL. In addition, mutants lacking sAPPL had numerous neurite branches with abnormal lamellate membrane structures (LMSs) and blebs, while no apoptosis was detected in these neurons. The abnormal neurite morphology was most likely due to the disorganization of the cytoskeleton, as shown by double staining of actin filaments and microtubules. Electro-physiologically, A-type K+ current was significantly enhanced, and spontaneous excitatory postsynaptic potentials (sEPSPs) were greatly increased in APPL mutants lacking sAPPL. Moreover, panneural overexpression of different forms of APPL protein generated different defects of wings and cuticle in adult flies. Taken together, our results suggest that both mAPPL and sAPPL play essential roles in the development of the central nervous system and nonneural tissues. (C) 2004 Wiley Periodicals, Inc.
学科主题	Neurosciences & Neurology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1932]
专题	上海神经科学研究所_神经所(总) 上海神经科学研究所_学习与记忆研究组
推荐引用方式 GB/T 7714	Guo, AK. Specific functions of Drosophila amyloid precursor-like protein in the development of nervous system and nonneural tissues[J]. JOURNAL OF NEUROBIOLOGY,2004,61(3):343-358.
APA	Guo, AK.(2004).Specific functions of Drosophila amyloid precursor-like protein in the development of nervous system and nonneural tissues.JOURNAL OF NEUROBIOLOGY,61(3),343-358.
MLA	Guo, AK."Specific functions of Drosophila amyloid precursor-like protein in the development of nervous system and nonneural tissues".JOURNAL OF NEUROBIOLOGY 61.3(2004):343-358.