Comprehensive Two-Dimensional Separation of Hydroxylated Polybrominated Diphenyl Ethers by Ultra-Performance Liquid Chromatography Coupled with Ion Mobility-Mass Spectrometry

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Comprehensive Two-Dimensional Separation of Hydroxylated Polybrominated Diphenyl Ethers by Ultra-Performance Liquid Chromatography Coupled with Ion Mobility-Mass Spectrometry
	Ma, Qiang ; Wang, Chao ; Bai, Hua ; Xi, Hai-Wei ; Xi, Guang-Cheng ; Ren, Xiao-Min ; Yang, Yu ; Guo, Liang-Hong
刊名	JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
	2011-10-01
卷号	22 期号:10 页码:1851—1861
中文摘要	A comprehensive two-dimensional system coupling ultra-performance liquid chromatography (UPLC) and ion mobility-mass spectrometry (IM-MS) has been applied for the separation and analysis of hydroxylated polybrominated diphenyl ethers (OH-PBDEs). A complex mixture containing 23 OH-PBDE congeners ranging from hydroxylated monobromodiphenyl ether (OH-monoBDE) to hydroxylated octabromodiphenyl ether (OH-octaBDE) was satisfactorily separated within 16 min of analysis time. The first-dimensional reversed-phase UPLC was performed on a sub-2 mu m BEH C(18) chromatographic column using acetonitrile-water gradient elution program with a flow rate ramp. It enabled excellent chromatographic separation for both between-class and within-class OH-PBDEs based on their differences in hydrophobicity. Following the pre-ionization resolution in the first dimension, the second-dimensional IM-MS employed a hybrid electrospray quadrupole ion mobility time-of-flight mass spectrometer and added an extra post-ionization separation for between-class OH-PBDE congeners on account of their relative mobility disparity during a very short period of 8.80 ms. The orthogonality of the developed two-dimensional system was evaluated with the correlation coefficient of 0.9665 and peak spreading angle of 14.87A degrees. The peak capacity of the system was calculated to be approximately 2 and 15 times higher than that of the two dimensions used alone, respectively. The two-dimensional separation plane also contributed to the removal of background interference ions and the enhanced confidence in the characterization of OH-PBDEs of interest.
WOS记录号	WOS:000295088400018
公开日期	2012-10-15
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/7000]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Ma, Qiang,Wang, Chao,Bai, Hua,et al. Comprehensive Two-Dimensional Separation of Hydroxylated Polybrominated Diphenyl Ethers by Ultra-Performance Liquid Chromatography Coupled with Ion Mobility-Mass Spectrometry[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2011,22(10):1851—1861.
APA	Ma, Qiang.,Wang, Chao.,Bai, Hua.,Xi, Hai-Wei.,Xi, Guang-Cheng.,...&Guo, Liang-Hong.(2011).Comprehensive Two-Dimensional Separation of Hydroxylated Polybrominated Diphenyl Ethers by Ultra-Performance Liquid Chromatography Coupled with Ion Mobility-Mass Spectrometry.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,22(10),1851—1861.
MLA	Ma, Qiang,et al."Comprehensive Two-Dimensional Separation of Hydroxylated Polybrominated Diphenyl Ethers by Ultra-Performance Liquid Chromatography Coupled with Ion Mobility-Mass Spectrometry".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 22.10(2011):1851—1861.