Study of stress effects of the rice (Oryza sativa L.) exposed to pesticides

	Study of stress effects of the rice (Oryza sativa L.) exposed to pesticides
	Zhou J(周佳) ; Wu ZM(吴泽明) ; Lu X(路鑫) ; Chang YW(常玉玮) ; Zhao CX(赵春霞) ; Xu GW(许国旺)
	2011
会议名称	37th international symposium on high performance liquid phase separations and related techniques
会议日期	2011-10-8
会议地点	大连
页码	493-0
通讯作者	许国旺
中文摘要	pesticide is widely employed to control the harmful insects and reduce the damage to crops. however, pesticide may have potential side effects to growing plants. in this study, rice (oryza sativa l.) was studied to determine whether the insecticide exposure has acute effects on the metabolome of plants. gas chromatography-mass spectrometry based metabolic profiling technique is used to collect metabolic information. the cultivar minghui86 was cultivated in field and treated with commercially used pesticides to simulate the field practice protocol, while the untreated rice (control) was planted in the adjacent region. leaves were collected at six different time points before or after pesticide treatment (on day 0.5, 1, 2, 4 and 7 post-treatment) in order to evaluate the metabolic time response to pesticide stress. and in considering of the growth induced-metabolic changes, the treated and untreated plants from the same sampling time were compared. principal component analysis (pca) was carried out to investigate the metabolic changing tendency and grouping. the trajectory based on the pca scores revealed the temporal changes of the rice metabolome. the metabolic response following the growth was observed in untreated samples during the sampling period. to discriminate the pesticide effects from the growth effects, the relative variation between treated and control groups was measured. all metabolites were subjected to qt-cluster analysis (according to the pearson correlation coefficients) to recognize the temporal patterns in relation to the pesticide treatment. the treated and untreated rice displayed different metabolic patterns over the sampling period, which implied that some metabolites may be affected under the pesticide stress. compared to the control, the treated rice leaves collected on day 2 after the pesticide spray showed greatly distinct metabolite profiles, especially. as for the cultivar minghui86, there were still significant metabolite differences between the treated and untreated groups after 7 days. it can be assumed that it will take more than 7 days to recover from the pesticide effects and returned to the level of untreated plants.
合作状况	墙报
会议主办者	中国化学会色谱专业委员会
会议录	proceeding of hplc 2011
会议录出版者	待补充
会议录出版地	待补充
学科主题	分析化学
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/116076]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Zhou J,Wu ZM,Lu X,et al. Study of stress effects of the rice (Oryza sativa L.) exposed to pesticides[C]. 见:37th international symposium on high performance liquid phase separations and related techniques. 大连. 2011-10-8.