PREPARATION MAGNETIC MESOPOROUS MATERIAL USED FOR SELECTIVE ENRICHMENT OF IMPORTANT CIS-DIOL-CONTAINING BIOMOLECULES

	PREPARATION MAGNETIC MESOPOROUS MATERIAL USED FOR SELECTIVE ENRICHMENT OF IMPORTANT CIS-DIOL-CONTAINING BIOMOLECULES
	Li H(李华) ; Shi XZ(石先哲) ; Lu X(路鑫) ; Xu GW(许国旺)
	2011
会议名称	14th beijing conference and exhibition on instrumental analysis
会议日期	2011-10-12
会议地点	北京
页码	527-1
通讯作者	许国旺
中文摘要	some cis-diol-containing biomolecules such as glycoproteins, modified nucleosides are closely linked with biological processes such as cellular recognition, proliferation. what’s more important, some of them are biomarkers of some illnesses such as malignant tumor, and the fluctuation of their content is closely related to the lesion progression. unfortunately, detection of the cis-diol-containing biomolecules is affected seriously by other compounds which coexist with them in body fluid abundantly, in addition most of the cis-diol-containing biomolecules are existing in trace amount. obviously detection is difficult so selective enrichment is necessary. in recent decades, great efforts have been paid to selective enrichment cis-diol-containing biomolecules. typically, commercialized agarose-bead -bound m-aminophenyl boronic acid is a kind of favorite spe stationary phase however it has to be controlled in alkaline media while capture and in acid media while release. as we know, the physiological ph is 7.4, so it is convenient if affinity can be realized in neutral ph ， and introducing a neighboring amino group to form wulff-type boronic acid or an electron-withdrawing group into the molecular can achieve such a goal. here we represent a kind of core-shell structure magnetic mesoporous material which is composed of mesoporous silica and magnetic nanoparticles. boronic acid affinity groups were adorned into the tunnel of mesoporous with 1,6-hexanediamine together to realize capture/release in physiological ph. mesoporous can supply size exclusion effects for cis-diol-containing biomolecules against other macromolecules and enlarge surface area at the same time. the magnetic nanoparticles could respond to external magnetic field rapidly so it will be convenient for the processes of pretreatment. tem showed that mesoporous was regularly arranged, and n2 adsorption demonstrated that surface area was about 698 m2/g and average pore diameter was about 2.2nm. ft-ir was used to prove the boronic acid groups have been introduced successfully. the mixture of modified nucleosides was used to test the selectivity of the new material.
合作状况	墙报
会议主办者	中国分析测试学会
会议录	proceeding of bceia 2011
会议录出版者	待补充
会议录出版地	待补充
学科主题	分析化学
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/116048]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Li H,Shi XZ,Lu X,et al. PREPARATION MAGNETIC MESOPOROUS MATERIAL USED FOR SELECTIVE ENRICHMENT OF IMPORTANT CIS-DIOL-CONTAINING BIOMOLECULES[C]. 见:14th beijing conference and exhibition on instrumental analysis. 北京. 2011-10-12.