Creation of Bioorthogonal Redox Systems Depending on Nicotinamide Flucytosine Dinucleotide

	Creation of Bioorthogonal Redox Systems Depending on Nicotinamide Flucytosine Dinucleotide
	Ji, Debin 1,2; Wang, Lei 1,2; Hou, Shuhua 1,2; Liu, Wujun 1,3; Wang, Jinxia 1; Wang, Qian 1,3; Zhao, Zongbao K.1,3
刊名	journal of the american chemical society
	2011-12-28
卷号	133 期号:51 页码:20857-20862
ISSN号	待补充
通讯作者	赵宗保
产权排序	1,1
中文摘要	creation of bioorthogonal redox systems depending on nicotinamide flucytosine dinucleotide
英文摘要	many enzymes catalyzing biological redox chemistry depend on the omnipresent cofactor, nicotinamide adenine dinucleotide (nad). nad is also involved in various nonredox processes. it remains challenging to disconnect one particular nad-dependent reaction from all others. here we present a bioorthogonal system that catalyzes the oxidative decarboxylation of l-malate with a dedicated abiotic cofactor, nicotinamide flucytosine dinucleotide (nfcd). by screening the multisite saturated mutagenesis libraries of the nad-dependent malic enzyme (me), we identified the mutant me-l310r/q401c, which showed excellent activity with nfcd, yet marginal activity with nad. we found that another synthetic cofactor, nicotinamide cytosine dinucleotide (ncd), also displayed similar activity with the me mutants. inspired by these observations, we mutated d-lactate dehydrogenase (dldh) and malate dehydrogenase (mdh) to dldh-v152r and mdh-l6r, respectively, and both mutants showed fully active with nfcd. when coupled with dldh-v152r, me-l310r/q401c required only a catalytic amount of nfcd to convert l-malate. our results opened the window to engineer bioorthogonal redox systems for a wide variety of applications in systems biology and synthetic biology.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	escherichia-coli ; directed evolution ; nad(+) transporter ; xylose reductase ; small molecules ; swiss-model ; specificity ; enzymes ; binding ; protein
收录类别	SCI
语种	英语
WOS记录号	WOS:000298571600037
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115835]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 3.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Ji, Debin,Wang, Lei,Hou, Shuhua,et al. Creation of Bioorthogonal Redox Systems Depending on Nicotinamide Flucytosine Dinucleotide[J]. journal of the american chemical society,2011,133(51):20857-20862.
APA	Ji, Debin.,Wang, Lei.,Hou, Shuhua.,Liu, Wujun.,Wang, Jinxia.,...&Zhao, Zongbao K..(2011).Creation of Bioorthogonal Redox Systems Depending on Nicotinamide Flucytosine Dinucleotide.journal of the american chemical society,133(51),20857-20862.
MLA	Ji, Debin,et al."Creation of Bioorthogonal Redox Systems Depending on Nicotinamide Flucytosine Dinucleotide".journal of the american chemical society 133.51(2011):20857-20862.