Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought

	Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought
	Shu, Liebo 1,3; Lou, Qiaojun 1; Ma, Chenfei 2; Ding, Wei 1; Zhou, Jia 2; Wu, Jinhong 1; Feng, Fangjun 1; Lu, Xin 2; Luo, Lijun 1,3; Xu, Guowang 2
刊名	proteomics
	2011-11-01
卷号	11 期号:21 页码:4122-4138
关键词	cDNA microarray Drought-induced stress Metabolites Oryza sativa L. Plant proteomics
ISSN号	待补充
通讯作者	许国旺
产权排序	2,3
中文摘要	genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought
英文摘要	we used proteomic analysis to determine the response of rice plant seedlings to drought-induced stress. the expression of 71 protein spots was significantly altered, and 60 spots were successfully identified. the greatest down-regulated protein functional category was translation. up-regulated proteins were mainly related to protein folding and assembly. additionally, many proteins involved in metabolism (e. g. carbohydrate metabolism) also showed differences in expression. cdna microarray and gc-ms analysis showed 4756 differentially expressed mrnas and 37 differentially expressed metabolites. once these data were integrated with the proteomic analysis, we were able to elucidate the metabolic pathways affected by drought-induced stress. these results suggest that increased energy consumption from storage substances occurred during drought. in addition, increased expression of the enzymes involved in anabolic pathways corresponded with an increase in the content of six amino acids. we speculated that energy conversion from carbohydrates and/or fatty acids to amino acids was increased. analysis of basic metabolism networks allowed us to understand how rice plants adjust to drought conditions.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods ; biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	oryza-sativa l. ; transgenic arabidopsis plants ; sucrose-phosphate synthase ; water-stress ; carbohydrate-metabolism ; abiotic stress ; maize leaves ; protein network ; osmotic-stress ; heat-shock
收录类别	SCI
语种	英语
WOS记录号	WOS:000297223200002
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115674]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Shanghai Agrobiol Gene Ctr, Shanghai 201106, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 3.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Shu, Liebo,Lou, Qiaojun,Ma, Chenfei,et al. Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought[J]. proteomics,2011,11(21):4122-4138.
APA	Shu, Liebo.,Lou, Qiaojun.,Ma, Chenfei.,Ding, Wei.,Zhou, Jia.,...&Mei, Hanwei.(2011).Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought.proteomics,11(21),4122-4138.
MLA	Shu, Liebo,et al."Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought".proteomics 11.21(2011):4122-4138.