Gas chromatography-mass spectrometric method for metabolic profiling of tobacco leaves

	Gas chromatography-mass spectrometric method for metabolic profiling of tobacco leaves
	Li, Yong 1; Pang, Tao 2; Li, Yanli 1; Wang, Xiaolin 1; Li, Qinghua 1,3; Lu, Xin 1; Xu, Guowang 1
刊名	journal of separation science
	2011-06-01
卷号	34 期号:12 页码:1447-1454
关键词	GC-MS Metabolic profiling Tobacco
ISSN号	待补充
通讯作者	许国旺
产权排序	1,1
中文摘要	gas chromatography-mass spectrometric method for metabolic profiling of tobacco leaves
英文摘要	a gas chromatography-mass spectrometric method was developed for profiling of tobacco leaves. the differentiation among tobacco leaves planted in two different regions was investigated. prior to analysis, the extraction solvent formulation was optimized and a combination of water, methanol and acetonitrile with a volume ratio of 3:1:1 was found to be optimal. the reproducibility of the method was satisfactory. kendall tau-b rank correlation coefficients were equal to 1 (p<0.05) for 82% of the resolved peaks (up to 95% of the overall peak areas), indicating the good response correlation. forty-four compounds including 9 saccharides, 9 alcohols, 9 amino acids, 16 organic acids and phosphoric acid were identified based on standard compounds. the method was successfully applied for profiling of tobacco leaves from zimbabwe and yunnan of china. our result revealed that levels of saccharides and their derivatives including xylose, ribose, fructose, glucose, turanose, xylitol and glyceric acid were more abundant while sucrose, glucitol and d-gluconic acid were less abundant in tobacco leaves from yunnan as compared to those from zimbabwe. amino acids such as l-alanine, l-tyrosine and l-threonine were found to be richer in zimbabwe tobacco than in yunnan tobacco.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	volatile organic-acids ; mainstream cigarette-smoke ; ms-based metabolomics ; gc-ms ; metabonomic analysis ; human urine ; hplc-ms ; derivatization ; microextraction ; optimization
收录类别	SCI
语种	英语
WOS记录号	WOS:000291986600011
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115673]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Yunnan Acad Tobacco Agr Sci, Yuxi, Peoples R China 3.Kunming Cigarette Factory Hongyun Tobacco Grp Co, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Li, Yong,Pang, Tao,Li, Yanli,et al. Gas chromatography-mass spectrometric method for metabolic profiling of tobacco leaves[J]. journal of separation science,2011,34(12):1447-1454.
APA	Li, Yong.,Pang, Tao.,Li, Yanli.,Wang, Xiaolin.,Li, Qinghua.,...&Xu, Guowang.(2011).Gas chromatography-mass spectrometric method for metabolic profiling of tobacco leaves.journal of separation science,34(12),1447-1454.
MLA	Li, Yong,et al."Gas chromatography-mass spectrometric method for metabolic profiling of tobacco leaves".journal of separation science 34.12(2011):1447-1454.