Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide

	Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide
	Zhang, Hongyan 1,2; Zhao, Xiaoming 1; Yang, Jinli 1; Yin, Heng 1; Wang, Wenxia 1; Lu, Hang 1; Du, Yuguang 1
刊名	plant cell reports
	2011-06-01
卷号	30 期号:6 页码:1153-1162
关键词	COS NO OIPK Defense-related enzyme Tobacco
ISSN号	0721-7714
通讯作者	赵小明 ; 杜昱光
产权排序	1,1
中文摘要	nitric oxide production and its functional link with oipk in tobacco defense response elicited by chitooligosaccharide
英文摘要	chitooligosaccharide (cos) or oligochitosan has been shown to induce tobacco defense responses which are connected with nitric oxide (no) and oipk (oligochitosan-induced ser/thr protein kinase). the aim of this study was to reveal the relationship between no production and oipk pathway in the defense response of tobacco elicited by cos. no generation was investigated by epidermal strip bioassay and fluorophore microscope using fluorophore diaminofluorescein diacetate (daf-2da). tobacco epidermal cells treated with cos resulted in production of no, which was first present in chloroplast, then in nucleus, finally in the whole cell; this no production was sensitive to no scavenger cptio and the mammalian no synthase (nos) inhibitor l-name, suggesting that nos-like enzyme maybe involved in no generation in tobacco epidermal cells. however, nos and nitrate reductase (nr, ec 1.6.6.1) inhibitors reduced no content in tobacco leaves by using no assay kit, suggesting both nos and nr were involved in no production in tobacco leaves. using a pharmacological approach and western blotting, we provide evidence that no acts upstream of oipk expression. no scavenger, nos inhibitor partly blocked the activation of oipk and the activities of several defense-related enzymes induced by cos; treatment with no donor sodium nitroprusside (snp) induced the activation of oipk and enhanced the defense systems. the results suggest that cos is able to induce no generation, which results in up-regulation the activities of some defense-related enzymes through an oipk-dependent or independent pathway.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	plant sciences
研究领域[WOS]	plant sciences
关键词[WOS]	plant-disease resistance ; s-nitrosylated proteins ; arabidopsis-thaliana ; in-vitro ; suspension-cultures ; nitrate reductase ; synthase activity ; innate immunity ; mosaic-virus ; no
收录类别	SCI
语种	英语
WOS记录号	WOS:000290542800020
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115510]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Hongyan,Zhao, Xiaoming,Yang, Jinli,et al. Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide[J]. plant cell reports,2011,30(6):1153-1162.
APA	Zhang, Hongyan.,Zhao, Xiaoming.,Yang, Jinli.,Yin, Heng.,Wang, Wenxia.,...&Du, Yuguang.(2011).Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide.plant cell reports,30(6),1153-1162.
MLA	Zhang, Hongyan,et al."Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide".plant cell reports 30.6(2011):1153-1162.