The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation

	The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation
	Gai, Qing-Qing 1; Qu, Feng 1; Zhang, Tao 1; Zhang, Yu-Kui 2
刊名	journal of chromatography a
	2011-06-03
卷号	1218 期号:22 页码:3489-3495
关键词	Molecularly imprinted polymer Magnetic particles Bovine serum albumin Protein separation
ISSN号	待补充
通讯作者	屈锋 ; 张玉奎
产权排序	2,4
中文摘要	the preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation
英文摘要	currently, small proteins imprinting are more reported since large proteins molecular imprinting faces challenge due to their bulk size and complex structure. in this work, bovine serum albumin (bsa) surface-imprinted magnetic polymer was successfully synthesized based on atomic transfer radical polymerization (atrp) method in the presence of common monomer (n-isopropylacrylamide) with the assistant of basic functional monomer (n-[3-(dimethylamino)propyl]-methacrylamide), which provides a achievable attempt for imprinting larger target proteins based on the atpr with the mild reaction conditions. the bsa-imprinted polymer exhibited higher adsorption capacity and selectivity to bsa over the non-imprinted polymer. competitive adsorption tests indicated the bsa-imprinted polymer had better selective adsorption and recognition properties to bsa in the mixture. the obtained bsa-imprinted polymer was applied to bovine serum, which also showed selectivity to bsa. in addition, a conventional aqueous two-phase solution of peg/sulphate was used as elution for adsorbed bsa, which was compared with common nacl elution. (c) 2011 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	submicrometer particles ; magnetic-susceptibility ; recognition property ; aqueous-media ; hydrogel ; microspheres ; lysozyme ; beads
收录类别	SCI
语种	英语
WOS记录号	WOS:000291132600011
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115325]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Gai, Qing-Qing,Qu, Feng,Zhang, Tao,et al. The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation[J]. journal of chromatography a,2011,1218(22):3489-3495.
APA	Gai, Qing-Qing,Qu, Feng,Zhang, Tao,&Zhang, Yu-Kui.(2011).The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation.journal of chromatography a,1218(22),3489-3495.
MLA	Gai, Qing-Qing,et al."The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation".journal of chromatography a 1218.22(2011):3489-3495.