Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins

	Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins
	Gai, Qingqing 1; Qu, Feng 1; Zhang, Tao 1; Zhang, Yukui 2
刊名	talanta
	2011-07-15
卷号	85 期号:1 页码:304-309
关键词	Carboxyl modified magnetic particles Aqueous two-phase extraction Protein Magnetic separation
ISSN号	待补充
通讯作者	屈锋 ; 张玉奎
产权排序	2,4
中文摘要	integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins
英文摘要	both of the magnetic particle adsorption and aqueous two-phase extraction (atpe) were simple, fast and low-cost method for protein separation. selective proteins adsorption by carboxyl modified magnetic particles was investigated according to protein isoelectric point, solution ph and ionic strength. aqueous two-phase system of peg/sulphate exhibited selective separation and extraction for proteins before and after magnetic adsorption. the two combination ways, magnetic adsorption followed by atpe and atpe followed by magnetic adsorption, for the separation of proteins mixture of lysozyme, bovine serum albumin, trypsin, cytochrome c and myloglobin were discussed and compared. the way of magnetic adsorption followed by atpe was also applied to human serum separation. (c) 2011 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	proteomic analysis ; systems ; purification ; copolymer ; lysozyme ; serum ; beads
收录类别	SCI
语种	英语
WOS记录号	WOS:000292848900041
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115324]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Gai, Qingqing,Qu, Feng,Zhang, Tao,et al. Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins[J]. talanta,2011,85(1):304-309.
APA	Gai, Qingqing,Qu, Feng,Zhang, Tao,&Zhang, Yukui.(2011).Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins.talanta,85(1),304-309.
MLA	Gai, Qingqing,et al."Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins".talanta 85.1(2011):304-309.