Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics

	Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics
	Sun, Liangliang 1,2; Tao, Dingyin 1,2; Han, Bin 1; Ma, Junfeng 1; Zhu, Guijie 1; Liang, Zhen 1; Shan, Yichu 1; Zhang, Lihua 1; Zhang, Yukui 1
刊名	analytical and bioanalytical chemistry
	2011-04-01
卷号	399 期号:10 页码:3387-3397
关键词	Ionic liquid 1-Butyl-3-methyl imidazolium tetrafluoroborate Integral membrane proteins Shotgun
ISSN号	待补充
通讯作者	张丽华
产权排序	1,1
中文摘要	ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics
英文摘要	the solubility and digestion efficiency are two crucial factors that might affect the identification of integral membrane proteins (imps). in this work, 1% (v/v) ionic liquid (il), 1-butyl-3-methyl imidazolium tetrafluoroborate (bmim bf(4)), added in nh(4)hco(3) buffer (ph 8.3), was applied as a sample preparation buffer for imps analysis. compared to the commonly used sodium dodecyl sulfate and methanol methods, the number of identified imps from rat brain by microcolumn reversed phase liquid chromatography (mu rplc)-electrospray ionization tandem mass spectrometry (esi-ms/ms) was improved by over three times, which might be due to the fact that bmim bf(4) offered high solubilizing ability for imps and good compatibility for tryptic digestion. furthermore, compared to rapigest and urea methods, with bmim bf(4) method, the number of identified imps from rat brain could be improved 25% and 80%, respectively, which might be contributed to the good solubilizing ability and high thermal stability of such il. with the sample treated by bmim bf(4) method, by 2d-nanoscx-rplc-esi-ms/ms, 1,450 non-redundant proteins and 7,978 unique peptides were identified from rat brain, and 418 proteins contained at least one predicted transmembrane domain, with false discovery rates of less than 1% for peptide identification, and at least two identified unique peptides per protein. all these results demonstrate that the bmim bf(4) method is of high potential for the large-scale identification of imps.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	desorption/ionization mass-spectrometry ; lc-ms/ms ; trypsin digestion ; plasma-membrane ; large-scale ; proteins ; extraction ; amino ; ph ; identification
收录类别	SCI
语种	英语
WOS记录号	WOS:000288398700008
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115311]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Sun, Liangliang,Tao, Dingyin,Han, Bin,et al. Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics[J]. analytical and bioanalytical chemistry,2011,399(10):3387-3397.
APA	Sun, Liangliang.,Tao, Dingyin.,Han, Bin.,Ma, Junfeng.,Zhu, Guijie.,...&Zhang, Yukui.(2011).Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics.analytical and bioanalytical chemistry,399(10),3387-3397.
MLA	Sun, Liangliang,et al."Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics".analytical and bioanalytical chemistry 399.10(2011):3387-3397.