Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis
	Wei, Xiumei 2; Yang, Jianmin 2; Yang, Dinglong 2; Xu, Jie 2; Liu, Xiangquan 2; Yang, Jialong 1; Fang, Jinghui 2; Qiao, Hongjin 2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012
卷号	32 期号:1 页码:178-185
关键词	Solen grandis PGRP Innate immunity Pattern recognition receptor Real-time PCR
ISSN号	1050-4648
通讯作者	Yang, JL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China. lxq6808@163.com ; jlyang@yic.ac.cn
产权排序	[Yang, Jialong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wei, Xiumei; Yang, Jianmin; Yang, Dinglong; Xu, Jie; Liu, Xiangquan; Fang, Jinghui; Qiao, Hongjin] Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China
中文摘要	Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk Solen grandis (designated as SgPGRP-S1 and SgPGRP-S2) were identified, and their expression patterns, both in tissues and toward three PAMPs stimulation, were then characterized. The full-length cDNA of SgPGRP-S1 and SgPGRP-S2 was 1672 and 1285 bp, containing an open reading frame (ORF) of 813 and 426 bp, respectively, and deduced amino acid sequences showed high similarity to other members of PGRP superfamily. Both SgPGRP-S1 and SgPGRP-S2 encoded a PGRP domain. The motif of Zn2+ binding sites and amidase catalytic sites were well conserved in SgPGRP-S1, but partially conserved in SgPGRP-S2. The two PGRPs exhibited different tissue expression pattern. SgPGRP-S1 was highly expressed in muscle and hepatopancreas, while SgPGRP-S2 was highly in gill and mantle. The mRNA expression of SgPGRP-S1 could be induced acutely by stimulation of PGN, and also moderately by beta-1,3-glucan, but not by LPS, while expression of SgPGRP-S2 was significantly upregulated (P < 0.01) when S. grandis was stimulated by all the three PAMPs, though the expression levels were relatively lower than SgPGRP-S1. Our results suggested SgPGRP-S1 and SgPGRP-S2 could serve as pattern recognition receptors (PRRs) involved in the immune recognition of S. grandis, and they might perform different functions in the immune defense against invaders.
英文摘要	Peptidoglycan recognition proteins (PGRPs) play crucial role in innate immunity for both invertebrates and vertebrates, owing to their prominent ability in detecting and eliminating invading bacteria. In the present study, two short PGRPs from mollusk Solen grandis (designated as SgPGRP-S1 and SgPGRP-S2) were identified, and their expression patterns, both in tissues and toward three PAMPs stimulation, were then characterized. The full-length cDNA of SgPGRP-S1 and SgPGRP-S2 was 1672 and 1285 bp, containing an open reading frame (ORF) of 813 and 426 bp, respectively, and deduced amino acid sequences showed high similarity to other members of PGRP superfamily. Both SgPGRP-S1 and SgPGRP-S2 encoded a PGRP domain. The motif of Zn2+ binding sites and amidase catalytic sites were well conserved in SgPGRP-S1, but partially conserved in SgPGRP-S2. The two PGRPs exhibited different tissue expression pattern. SgPGRP-S1 was highly expressed in muscle and hepatopancreas, while SgPGRP-S2 was highly in gill and mantle. The mRNA expression of SgPGRP-S1 could be induced acutely by stimulation of PGN, and also moderately by beta-1,3-glucan, but not by LPS, while expression of SgPGRP-S2 was significantly upregulated (P < 0.01) when S. grandis was stimulated by all the three PAMPs, though the expression levels were relatively lower than SgPGRP-S1. Our results suggested SgPGRP-S1 and SgPGRP-S2 could serve as pattern recognition receptors (PRRs) involved in the immune recognition of S. grandis, and they might perform different functions in the immune defense against invaders. (C) 2011 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	L-ALANINE AMIDASE ; INNATE IMMUNITY ; DROSOPHILA-MELANOGASTER ; BACTERIAL-INFECTION ; CRASSOSTREA-GIGAS ; CHLAMYS-FARRERI ; PACIFIC OYSTER ; ACTIVATION ; RECEPTOR ; DEFENSE
收录类别	SCI
资助信息	Program of Agriculture Thoroughbred Project, Shandong province, China
原文出处	http://www.sciencedirect.com/science/journal/10504648
语种	英语
WOS记录号	WOS:000299979100021
公开日期	2012-06-13
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5566]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室海岸带生物资源高效利用研究与发展中心
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Shandong Marine Fisheries Res Inst, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Wei, Xiumei,Yang, Jianmin,Yang, Dinglong,et al. Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(1):178-185.
APA	Wei, Xiumei.,Yang, Jianmin.,Yang, Dinglong.,Xu, Jie.,Liu, Xiangquan.,...&Qiao, Hongjin.(2012).Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis.FISH & SHELLFISH IMMUNOLOGY,32(1),178-185.
MLA	Wei, Xiumei,et al."Molecular cloning and mRNA expression of two peptidoglycan recognition protein (PGRP) genes from mollusk Solen grandis".FISH & SHELLFISH IMMUNOLOGY 32.1(2012):178-185.