Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge
	Liu, Wuxing 1; Wang, Xiaobing 1; Wu, Longhua 1; Chen, Mengfang 1; Tu, Chen1 ; Luo, Yongming1,2 ; Christie, Peter 3
刊名	CHEMOSPHERE
	2012-06-01
卷号	87 期号:10 页码:1105-1110
关键词	Oily sludge Biosurfactants Oil recovery Bacillus amyloliquefaciens
ISSN号	0045-6535
通讯作者	Christie, P (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. ymluo@issas.ac.cn
产权排序	[Liu, Wuxing; Wang, Xiaobing; Wu, Longhua; Chen, Mengfang; Tu, Chen; Luo, Yongming] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China; [Luo, Yongming] Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Christie, Peter] Agri Food & Biosci Inst, Agri Environm Branch, Belfast BT9 5PX, Antrim, North Ireland
中文摘要	Over 100 biosurfactant-producing microorganisms were isolated from oily sludge and petroleum-contaminated soil from Shengli oil field in north China. Sixteen of the bacterial isolates produced biosurfactants and reduced the surface tension of the growth medium from 71 to <30 mN m(-1) after 72 h of growth. These bacteria were used to treat oily sludge and the recovery efficiencies of oil from oily sludge were determined. The oil recovery efficiencies of different isolates ranged from 39% to 88%. Bacterial isolate BZ-6 was found to be the most efficient strain and the three phases (oil, water and sediment) were separated automatically after the sludge was treated with the culture medium of BZ-6. Based on morphological, physiological characteristics and molecular identification, isolate BZ-6 was identified as Bacillus amyloliquefaciens. The biosurfactant produced by isolate BZ-6 was purified and analyzed by high performance liquid chromatography-electrospray ionization tandem mass spectrometry. There were four ion peaks representing four different fengycin A homologues.
英文摘要	Over 100 biosurfactant-producing microorganisms were isolated from oily sludge and petroleum-contaminated soil from Shengli oil field in north China. Sixteen of the bacterial isolates produced biosurfactants and reduced the surface tension of the growth medium from 71 to <30 mN m(-1) after 72 h of growth. These bacteria were used to treat oily sludge and the recovery efficiencies of oil from oily sludge were determined. The oil recovery efficiencies of different isolates ranged from 39% to 88%. Bacterial isolate BZ-6 was found to be the most efficient strain and the three phases (oil, water and sediment) were separated automatically after the sludge was treated with the culture medium of BZ-6. Based on morphological, physiological characteristics and molecular identification, isolate BZ-6 was identified as Bacillus amyloliquefaciens. The biosurfactant produced by isolate BZ-6 was purified and analyzed by high performance liquid chromatography-electrospray ionization tandem mass spectrometry. There were four ion peaks representing four different fengycin A homologues. (C) 2012 Published by Elsevier Ltd.
学科主题	Environmental Sciences & Ecology
研究领域[WOS]	Environmental Sciences & Ecology
关键词[WOS]	BIOSURFACTANT PRODUCTION ; CONTAMINATED SOILS ; CRUDE-OIL ; MICROORGANISMS ; REMEDIATION ; SUBTILIS
收录类别	SCI
资助信息	Chinese Academy of Sciences[KSCX2-YW-G-053, KZCX2-YW-Q02-02]; National Natural Science Foundation of China[41001182]; Environmental Protection Public Welfare Special Fund for Scientific Research[201009015]
原文出处	http://www.springerlink.com/content/0923-9820/
语种	英语
WOS记录号	WOS:000303292800004
公开日期	2012-06-13
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5508]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_污染过程与控制实验室
作者单位	1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Agri Food & Biosci Inst, Agri Environm Branch, Belfast BT9 5PX, Antrim, North Ireland
推荐引用方式 GB/T 7714	Liu, Wuxing,Wang, Xiaobing,Wu, Longhua,et al. Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge[J]. CHEMOSPHERE,2012,87(10):1105-1110.
APA	Liu, Wuxing.,Wang, Xiaobing.,Wu, Longhua.,Chen, Mengfang.,Tu, Chen.,...&Christie, Peter.(2012).Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge.CHEMOSPHERE,87(10),1105-1110.
MLA	Liu, Wuxing,et al."Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge".CHEMOSPHERE 87.10(2012):1105-1110.