Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection

	Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection
	Yang X ; Li T ; Li BL ; Wang EK
刊名	analyst
	2010
卷号	135 期号:1 页码:71-75
关键词	COLORIMETRIC DETECTION PEROXIDASE-ACTIVITY AMPLIFIED ANALYSIS DNAZYME ION THROMBIN APTAMERS SENSOR OLIGONUCLEOTIDE NANOPARTICLES
ISSN号	0003-2654
通讯作者	wang ek
中文摘要	a novel visual method for k+ detection is developed utilizing a k+-sensitive g-quadruplex dna named ps5.m as the sensing element. in the absence of k+, ps5.m is in a random coil state and does not bind hemin. upon addition of k+, ps5.m folds into the quadruplex structure stabilized by k+. such quadruplex structure is able to bind hemin to form the hemin-g-quadruplex dnazyme that catalyzes the h2o2-mediated oxidation of colorless 3,3',5,5'-tetramethylbenzidine to the blue product. under the optimal conditions, the color change can be clearly observed with the naked eye in the concentration range from 2 to 1000 mm. through this facile approach, k+ can be detected down to 2 mu m, with high selectivity against other cations. in view of its simplicity, sensitivity and specificity, our developed visual method for k+ detection would potentially be utilized for bioassays and k+-sensitive test paper.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000272711400008
公开日期	2012-06-07
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/43995]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Yang X,Li T,Li BL,et al. Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection[J]. analyst,2010,135(1):71-75.
APA	Yang X,Li T,Li BL,&Wang EK.(2010).Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection.analyst,135(1),71-75.
MLA	Yang X,et al."Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection".analyst 135.1(2010):71-75.