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	Identification of Cd-Responsive Genes of Solanum nigrum Seedlings Through Differential Display
	Xu J.
	2009
关键词	Cadmium toxicity Nonradioactive differential display Hyperaccumulator glutamine-synthetase oxidative stress cadmium stress metal plants hyperaccumulator accumulation expression l. phytoremediation
英文摘要	Solanum nigrum is known as a Cd-hyperaccumulator: Its ability to accumulate large amounts of cadmium in leaves affords its designation as an effective phytoremediator. To identify Cd-responsive genes in S. nigrum, a nonradioactive differential display reverse transcription polymerase chain reaction technique was applied to isolate of genes whose transcription was altered in seedlings under Cd stress. A total of 48 DD bands were identified; from these bands, fragments corresponding to seven cDNAs were cloned. Reverse Northern dot-blot analysis confirmed the different expression patterns of these genes under cadmium toxicity. The homology analysis revealed that five of these cDNAs had a clear identity to Solanum species, and putative functions were assigned, including calmodulin, ascorbate peroxidase, catalase, glutamylcysteine synthetase, and iron-regulated transporter. Finally, the involvement of these genes in heavy metal tolerance is discussed.
出处	Plant Molecular Biology Reporter
卷	27
期	4
页	563-569
收录类别	SCI
语种	英语
ISSN号	0735-9640
内容类型	SCI/SSCI论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/22712]
专题	地理科学与资源研究所_历年回溯文献
推荐引用方式 GB/T 7714	Xu J.. Identification of Cd-Responsive Genes of Solanum nigrum Seedlings Through Differential Display. 2009.

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