Nanogold-assisted multi-round polymerase to chain reaction (PCR)

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	Nanogold-assisted multi-round polymerase to chain reaction (PCR)
	Pan, JK ; Li, HK ; Cao, XY ; Huang, JH ; Zhang, XD ; Fan, CH(樊春海） ; Hu, J（胡钧）
刊名	JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
	2007
卷号	7 期号:12 页码:4428
ISSN号	1533-4880
通讯作者	Zhang, XD (reprint author), Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Nanobiol Lab, Bio X Life Sci Res Ctr, Shanghai 200240, Peoples R China
英文摘要	We have previously demonstrated that nanogold effectively enhances the specificity and yield of error-prone two-round polymerase chain reaction (PCR). Here we reported that, with the assistance of nanogold, we could perform multi-round PCR. In the presence of appropriate amount of 10 nm nanogold, we could obtain the target product even after six rounds of PCR, as manifested by a single bright band in gel electrophoresis (1% agarose). In fact, we could still observe the target band even at the 7th round of PCR, which nevertheless was accompanied by smearing bands (non-specific amplification). In contrast, in the absence of nanogold, the target band was completely lost only after four rounds of amplification. This marked difference in the performance of multi-round PCR clearly showed that nanogold was a powerful enhancer for PC,R. More importantly, with this nanogold-assisted multi-round PCR, it might be possible to produce a large amount of target DNA, or to amply very low copies of genomic DNA from rare sources.
学科主题	Physics
收录类别	SCI
语种	英语
WOS记录号	WOS:000252511300026
公开日期	2012-04-18
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/7783]
专题	上海应用物理研究所_中科院上海应用物理研究所2004-2010年
推荐引用方式 GB/T 7714	Pan, JK,Li, HK,Cao, XY,et al. Nanogold-assisted multi-round polymerase to chain reaction (PCR)[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2007,7(12):4428.
APA	Pan, JK.,Li, HK.,Cao, XY.,Huang, JH.,Zhang, XD.,...&Hu, J.(2007).Nanogold-assisted multi-round polymerase to chain reaction (PCR).JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,7(12),4428.
MLA	Pan, JK,et al."Nanogold-assisted multi-round polymerase to chain reaction (PCR)".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 7.12(2007):4428.