Design of an Oligonucleotide-Incorporated Nonfouling Surface and Its Application in Electrochemical DNA Sensors for Highly Sensitive and Sequence-Specific Detection of Target DNA

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2004-2010年

	Design of an Oligonucleotide-Incorporated Nonfouling Surface and Its Application in Electrochemical DNA Sensors for Highly Sensitive and Sequence-Specific Detection of Target DNA
	Zhang, J(张炯） ; Lao, RJ ; Song, SP（宋世平） ; Yan, ZY ; Fan, CH（樊春海）
刊名	ANALYTICAL CHEMISTRY
	2008
卷号	80 期号:23 页码:9029
ISSN号	0003-2700
通讯作者	Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
英文摘要	In this work, we report a novel electrochemical DNA sensor based on a nonfouling monolayer structure self-assembled at gold surfaces. Self-assembled monolayers (SAMs) with oligo(ethylene glycol) (OEG)-terminated thiols are known to be highly protein-resistant and effectively repel nonspecific adsorption. We found that a mixed SAM structure incorporating thiolated oligonucleotides and OEG thiols (SH-DNA/OEG) exhibited the similar nonfouling feature. More importantly, it allowed facile electron transfer across the monolayer and thus was fully compatible with electrochemical detection. On the basis of this SH-DNA/OEG platform, we developed a sandwich-type electrochemical sensor for the sequence-specific detection of DNA targets. This sensor was able to detect as little as 1 pM target DNA even in the presence of complicated biological fluids such as human serum. We also employed this sensor to directly detect a polymerase chain reaction (PCR) amplicon from the genomic DNA of Escherichia coli K12, which led to a very low detect limit of 60 fg (similar to 10 copies).
学科主题	Physics
收录类别	SCI
语种	英语
WOS记录号	WOS:000261335600070
公开日期	2012-04-18
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/7688]
专题	上海应用物理研究所_中科院上海应用物理研究所2004-2010年
推荐引用方式 GB/T 7714	Zhang, J,Lao, RJ,Song, SP,et al. Design of an Oligonucleotide-Incorporated Nonfouling Surface and Its Application in Electrochemical DNA Sensors for Highly Sensitive and Sequence-Specific Detection of Target DNA[J]. ANALYTICAL CHEMISTRY,2008,80(23):9029.
APA	Zhang, J,Lao, RJ,Song, SP,Yan, ZY,&Fan, CH.(2008).Design of an Oligonucleotide-Incorporated Nonfouling Surface and Its Application in Electrochemical DNA Sensors for Highly Sensitive and Sequence-Specific Detection of Target DNA.ANALYTICAL CHEMISTRY,80(23),9029.
MLA	Zhang, J,et al."Design of an Oligonucleotide-Incorporated Nonfouling Surface and Its Application in Electrochemical DNA Sensors for Highly Sensitive and Sequence-Specific Detection of Target DNA".ANALYTICAL CHEMISTRY 80.23(2008):9029.