Characterization of a chestnut FLORICAULA/LEAFY homologous gene

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 资源植物与生物技术所级重点实验室

	Characterization of a chestnut FLORICAULA/LEAFY homologous gene
	Liu, Tao 1; Hu, Yun-qian 2; Li, Xiao-xian 2
刊名	AFRICAN JOURNAL OF BIOTECHNOLOGY
	2011-05-16
卷号	10 期号:20 页码:3978-3985
关键词	Chestnut homologue FLORICAULA/LEAFY
ISSN号	1684-5315
通讯作者	Liu, T (reprint author), Yunnan Agr Univ, Fac Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China
英文摘要	The FLORICAULA/LEAFY (FLO/LFY) homologues' genes are necessary for normal flower development and play a key role in diverse angiosperm species. In this paper, an orthologue of FLORICAULA/LEAFY, CmLFY (chestnut FLO/LFY), was isolated from the inflorescence of chestnut trees. Its expression was detected in various tissues. Furthermore, the flowering effectiveness of the gene was assessed with transgenic Arabidopsis. CmLFY protein showed a high degree of similarity to PEAFLO (78%), which is a homologue of FLO/LFY from pea. RT-PCR analysis showed that, CmLFY expressed at high levels in inflorescences, but not in young leaves, fruits or stems. The transgenic Arabidopsis with over-expressed CmLFY showed accelerated flowering, which supports that CmLFY encodes a functional orthologue of the FLORICAULA/LEAFY genes of angiosperms despite its sequence divergence. These results suggest that CmLFY is involved in inflorescence development in chestnut.
学科主题	Biotechnology & Applied Microbiology
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	FLORAL MERISTEM IDENTITY ; FLO/LFY HOMOLOG ; ARABIDOPSIS ; LEAFY ; FLOWER ; EXPRESSION ; CLONING ; OVEREXPRESSION ; INITIATION ; ORTHOLOG
收录类别	SCI
资助信息	Faculty of Agronomy and Biotechnology[SJCX09012]; National Natural Science Foundation of China (NSFC)[30800062]
语种	英语
WOS记录号	WOS:000294612200004
公开日期	2012-04-10
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/5309]
专题	昆明植物研究所_资源植物与生物技术所级重点实验室
作者单位	1.Yunnan Agr Univ, Fac Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Liu, Tao,Hu, Yun-qian,Li, Xiao-xian. Characterization of a chestnut FLORICAULA/LEAFY homologous gene[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(20):3978-3985.
APA	Liu, Tao,Hu, Yun-qian,&Li, Xiao-xian.(2011).Characterization of a chestnut FLORICAULA/LEAFY homologous gene.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(20),3978-3985.
MLA	Liu, Tao,et al."Characterization of a chestnut FLORICAULA/LEAFY homologous gene".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.20(2011):3978-3985.