Application of image alignment and time averaging methods in AFM detection for single DNA molecules | |
Wang, CM ; Sun, JL ; Long, F ; Zhao, J ; Hu, J | |
刊名 | CHINESE SCIENCE BULLETIN |
2010 | |
卷号 | 55期号:16页码:6 |
关键词 | image alignment DNA AFM time averaging SNR |
ISSN号 | 1001-6538 |
通讯作者 | Sun, JL (reprint author), Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China |
英文摘要 | Enhancement of the SNR (signal to noise ratio) in single-molecule imaging is significantly important for improving image resolution and distinguishing the fine structures of single molecules at a higher precision level. Image processing techniques have demonstrated the remarkable capability to improve the SNR and the resolution level by breaking through some inherent limitations unresolved by instrument hardware optimization. In this paper, we focus on single-biomolecule imaging using atomic force microscopy (AFM), a unique method in separated single-biomolecule imaging, and there was few suitable image processing techniques reported to increase the SNR of a single molecule's AFM image. With the typical samples of separately dispersed DNA molecules, we replaced the classified averaging method, which is usually used when the molecules' structure can be easily and repeatedly prepared, with the time averaging method to improve the SNR in a single molecule's AFM imaging. Combining the time averaging technique with the image alignment method for the series of time-lapse AFM images of a single DNA molecule, we were able to improve the image's SNR and recover some buried signals from the background noises. Furthermore, the fine structures of single molecules can potentially be further resolved if other image processing techniques are applied collaboratively with some newly developed imaging methods like Vibrating Mode Scanning Polarization Force Microscopy (VSPFM), and such combination will also be advantageous for the manipulation of single-biomolecules by AFM. In addition, the proposed algorithms for the estimations of drift, distortion and SNR are applicable for quantitative status characterization of AFM imaging. |
学科主题 | Science & Technology - Other Topics |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000278704200007 |
公开日期 | 2012-04-11 |
内容类型 | 期刊论文 |
源URL | [http://ir.sinap.ac.cn/handle/331007/6840] |
专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
推荐引用方式 GB/T 7714 | Wang, CM,Sun, JL,Long, F,et al. Application of image alignment and time averaging methods in AFM detection for single DNA molecules[J]. CHINESE SCIENCE BULLETIN,2010,55(16):6. |
APA | Wang, CM,Sun, JL,Long, F,Zhao, J,&Hu, J.(2010).Application of image alignment and time averaging methods in AFM detection for single DNA molecules.CHINESE SCIENCE BULLETIN,55(16),6. |
MLA | Wang, CM,et al."Application of image alignment and time averaging methods in AFM detection for single DNA molecules".CHINESE SCIENCE BULLETIN 55.16(2010):6. |
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