A rapid and sensitive "add-mix-measure" assay for multiple proteinases based on one gold nanoparticle-peptide-fluorophore conjugate

	A rapid and sensitive "add-mix-measure" assay for multiple proteinases based on one gold nanoparticle-peptide-fluorophore conjugate
	Wang XH ; Geng J ; Miyoshi D ; Ren JS ; Sugimoto N ; Qu XG
刊名	biosensors & bioelectronics
	2010
卷号	26 期号:2 页码:743-747
关键词	PANCREAS TRANSPLANT PATIENTS PROTEASE ACTIVITY TRYPSIN DUODENOCYSTOSTOMIES PROBES
ISSN号	0956-5663
通讯作者	qu xg
中文摘要	we report here a homogeneous fluorometric method for the assays of multiple proteinases based on one aunps-peptide-fluorophore conjugate as the substrate. the peptide portion of conjugates has cleavage sites for multiple proteinases. once nanoprobes are fabricated by end-immobilizing fluorophore labeled peptide onto the surface of gold nanoparticles, proteinase is introduced and enzymatic cleavage of residues in the peptide portion of the conjugates occurs as a consequence of the specific substrate recognition by the proteinase, manifesting in the form of strong fluorescence signal recovery. the method offers a sensitive and rapid evaluation of proteinases activity operating either in an endpoint or real-time format. the "add-mix-measure" format of this method eliminates tedious nanoprobes processing, significantly reduces the assay time and makes it have more generality. moreover, this assay can be used to detect proteinase activity in biological media. the developed assay is ideal for industrial routine tests, clinical assay and high-throughput screening proteinase inhibitors. as far as we know, this is the first report that a single substrate can be used to assess multiple proteinases which recognize different cleavage motifs. (c) 2010 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
公开日期	2012-04-25
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/43322]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang XH,Geng J,Miyoshi D,et al. A rapid and sensitive "add-mix-measure" assay for multiple proteinases based on one gold nanoparticle-peptide-fluorophore conjugate[J]. biosensors & bioelectronics,2010,26(2):743-747.
APA	Wang XH,Geng J,Miyoshi D,Ren JS,Sugimoto N,&Qu XG.(2010).A rapid and sensitive "add-mix-measure" assay for multiple proteinases based on one gold nanoparticle-peptide-fluorophore conjugate.biosensors & bioelectronics,26(2),743-747.
MLA	Wang XH,et al."A rapid and sensitive "add-mix-measure" assay for multiple proteinases based on one gold nanoparticle-peptide-fluorophore conjugate".biosensors & bioelectronics 26.2(2010):743-747.