Screening Lectin-Binding Specificity of Bacterium by Lectin Microarray with Gold Nanoparticle Probes

	Screening Lectin-Binding Specificity of Bacterium by Lectin Microarray with Gold Nanoparticle Probes
	Geo JQ ; Liu DJ ; Wang ZX
刊名	analytical chemistry
	2010
卷号	82 期号:22 页码:9240-9247
关键词	CARBOHYDRATE-PROTEIN INTERACTIONS ANGUILLA-ANGUILLA AGGLUTININ ESCHERICHIA-COLI ULEX-EUROPAEUS LIGANDS IDENTIFICATION RECOGNITION BIOSENSOR PATHOGENS AFFINITY
ISSN号	0003-2700
通讯作者	wang zx
中文摘要	to develop a novel high-throughput tool for monitoring specific affinity of microbes with lectins, a kind of lectin microarray has been fabricated by immobilizing lectins on epoxide-derivatized glass slides and used to capture microbes. the capturing events are marked by attachment of lectin-conjugated gold nanoparticles followed by silver deposition to enhance the resonance fight scattering (rls) of the particles. the interactions of 16 lectins with four bacteria and one fungus were profiled by this approach. we demonstrated that the gold-nanoparticle-labeled array was suitable for identifying the binding affinity of lectin with bacterium, as well as determining the bacterium with high sensitivity. more importantly, we found that the growth of microbial strains in different culture media resulted in significant changes in their binding affinities with lectins, which might be important to the pathogenesis of the organisms.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000284080500018
公开日期	2012-04-25
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/43310]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Geo JQ,Liu DJ,Wang ZX. Screening Lectin-Binding Specificity of Bacterium by Lectin Microarray with Gold Nanoparticle Probes[J]. analytical chemistry,2010,82(22):9240-9247.
APA	Geo JQ,Liu DJ,&Wang ZX.(2010).Screening Lectin-Binding Specificity of Bacterium by Lectin Microarray with Gold Nanoparticle Probes.analytical chemistry,82(22),9240-9247.
MLA	Geo JQ,et al."Screening Lectin-Binding Specificity of Bacterium by Lectin Microarray with Gold Nanoparticle Probes".analytical chemistry 82.22(2010):9240-9247.