A Novel Cyanophage with a Cyanobacterial Nonbleaching Protein A Gene in the Genome

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	A Novel Cyanophage with a Cyanobacterial Nonbleaching Protein A Gene in the Genome
	Gao, E-Bin; Gui, Jian-Fang; Zhang, Qi-Ya
刊名	JOURNAL OF VIROLOGY
	2012
卷号	86 期号:1 页码:236-245
ISSN号	0022-538X
通讯作者	Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	A cyanophage, PaV-LD, has been isolated from harmful filamentous cyanobacterium Planktothrix agardhii in Lake Donghu, a shallow freshwater lake in China. Here, we present the cyanophage's genomic organization and major structural proteins. The genome is a 95,299-bp-long, linear double-stranded DNA and contains 142 potential genes. BLAST searches revealed 29 proteins of known function in cyanophages, cyanobacteria, or bacteria. Thirteen major structural proteins ranging in size from 27 kDa to 172 kDa were identified by SDS-PAGE and mass-spectrometric analysis. The genome lacks major genes that are necessary to the tail structure, and the tailless PaV-LD has been confirmed by an electron microscopy comparison with other tail cyanophages and phages. Phylogenetic analysis of the major capsid proteins also reveals an independent branch of PaV-LD that is quite different from other known tail cyanophages and phages. Moreover, the unique genome carries a nonbleaching protein A (NblA) gene (open reading frame [ORF] 022L), which is present in all phycobilisome-containing organisms and mediates phycobilisome degradation. Western blot detection confirmed that 022L was expressed after PaV-LD infection in the host filamentous cyanobacterium. In addition, its appearance was companied by a significant decline of phycocyanobilin content and a color change of the cyanobacterial cells from blue-green to yellow-green. The biological function of PaV-LD nblA was further confirmed by expression in a model cyanobacterium via an integration platform, by spectroscopic analysis and electron microscopy observation. The data indicate that PaV-LD is an exceptional cyanophage of filamentous cyanobacteria, and this novel cyanophage will also provide us with a new vision of the cyanophage-host interactions.
英文摘要	A cyanophage, PaV-LD, has been isolated from harmful filamentous cyanobacterium Planktothrix agardhii in Lake Donghu, a shallow freshwater lake in China. Here, we present the cyanophage's genomic organization and major structural proteins. The genome is a 95,299-bp-long, linear double-stranded DNA and contains 142 potential genes. BLAST searches revealed 29 proteins of known function in cyanophages, cyanobacteria, or bacteria. Thirteen major structural proteins ranging in size from 27 kDa to 172 kDa were identified by SDS-PAGE and mass-spectrometric analysis. The genome lacks major genes that are necessary to the tail structure, and the tailless PaV-LD has been confirmed by an electron microscopy comparison with other tail cyanophages and phages. Phylogenetic analysis of the major capsid proteins also reveals an independent branch of PaV-LD that is quite different from other known tail cyanophages and phages. Moreover, the unique genome carries a nonbleaching protein A (NblA) gene (open reading frame [ORF] 022L), which is present in all phycobilisome-containing organisms and mediates phycobilisome degradation. Western blot detection confirmed that 022L was expressed after PaV-LD infection in the host filamentous cyanobacterium. In addition, its appearance was companied by a significant decline of phycocyanobilin content and a color change of the cyanobacterial cells from blue-green to yellow-green. The biological function of PaV-LD nblA was further confirmed by expression in a model cyanobacterium via an integration platform, by spectroscopic analysis and electron microscopy observation. The data indicate that PaV-LD is an exceptional cyanophage of filamentous cyanobacteria, and this novel cyanophage will also provide us with a new vision of the cyanophage-host interactions.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Virology
研究领域[WOS]	Virology
关键词[WOS]	LACTOCOCCAL BACTERIOPHAGE TP901-1 ; MARINE SYNECHOCOCCUS STRAINS ; MYCOBACTERIOPHAGE GENOMES ; PHOTOSYNTHESIS GENES ; EFFICIENT INFECTION ; TAIL PROTEINS ; PHAGE ; PROCHLOROCOCCUS ; SEQUENCE ; DIVERSITY
收录类别	SCI
资助信息	National Major Basic Research Program[2010CB126303, 2009CB118704]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; FEBL[2008FBZ15, 2008FBZ16]
语种	英语
WOS记录号	WOS:000298347700023
公开日期	2012-04-01
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16739]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Gao, E-Bin,Gui, Jian-Fang,Zhang, Qi-Ya. A Novel Cyanophage with a Cyanobacterial Nonbleaching Protein A Gene in the Genome[J]. JOURNAL OF VIROLOGY,2012,86(1):236-245.
APA	Gao, E-Bin,Gui, Jian-Fang,&Zhang, Qi-Ya.(2012).A Novel Cyanophage with a Cyanobacterial Nonbleaching Protein A Gene in the Genome.JOURNAL OF VIROLOGY,86(1),236-245.
MLA	Gao, E-Bin,et al."A Novel Cyanophage with a Cyanobacterial Nonbleaching Protein A Gene in the Genome".JOURNAL OF VIROLOGY 86.1(2012):236-245.