Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae

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	Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae
	Li, Xuemei 1,2; Yu, Yuhe 1; Feng, Weisong 1; Yan, Qingyun 1; Gong, Yingchun 1
刊名	JOURNAL OF MICROBIOLOGY
	2012-02-01
卷号	50 期号:1 页码:29-37
关键词	intestinal microbiota denaturing gradient gel electrophoresis freshwater fish larvae host species effects
ISSN号	1225-8873
通讯作者	Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
中文摘要	We investigated the influence of host species on intestinal microbiota by comparing the gut bacterial community structure of four cohabitating freshwater fish larvae, silver carp, grass carp, bighead carp, and blunt snout bream, using denaturing gradient gel electrophoresis (DGGE) of the amplified 16S and 18S rRNA genes. Similarity clustering indicated that the intestinal microbiota derived from these four fish species could be divided into four groups based on 16S rRNA gene similarity, whereas the eukaryotic 18S rRNA genes showed no distinct groups. The water sample from the shared environment contained microbiota of an independent group as indicated by both 16S and 18S rRNA genes segments. The bacterial community structures were visualized using rank-abundance plots fitted with linear regression models. Results showed that the intestinal bacterial evenness was significantly different between species (P<0.05) and between species and the water sample (P<0.01). Thirty-five relatively dominant bands in DGGE patterns were sequenced and grouped into five major taxa: Proteobacteria (26), Actinobacteria (5), Bacteroidetes (1), Firmicutes (2), and Cyanobacterial (1). Six eukaryotes were detected by sequencing 18S rRNA genes segments. The present study suggests that the intestines of the four fish larvae, although reared in the same environment, contained distinct bacterial populations, while intestinal eukaryotic microorganisms were almost identical.
英文摘要	We investigated the influence of host species on intestinal microbiota by comparing the gut bacterial community structure of four cohabitating freshwater fish larvae, silver carp, grass carp, bighead carp, and blunt snout bream, using denaturing gradient gel electrophoresis (DGGE) of the amplified 16S and 18S rRNA genes. Similarity clustering indicated that the intestinal microbiota derived from these four fish species could be divided into four groups based on 16S rRNA gene similarity, whereas the eukaryotic 18S rRNA genes showed no distinct groups. The water sample from the shared environment contained microbiota of an independent group as indicated by both 16S and 18S rRNA genes segments. The bacterial community structures were visualized using rank-abundance plots fitted with linear regression models. Results showed that the intestinal bacterial evenness was significantly different between species (P<0.05) and between species and the water sample (P<0.01). Thirty-five relatively dominant bands in DGGE patterns were sequenced and grouped into five major taxa: Proteobacteria (26), Actinobacteria (5), Bacteroidetes (1), Firmicutes (2), and Cyanobacterial (1). Six eukaryotes were detected by sequencing 18S rRNA genes segments. The present study suggests that the intestines of the four fish larvae, although reared in the same environment, contained distinct bacterial populations, while intestinal eukaryotic microorganisms were almost identical.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	GRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; TROUT ONCORHYNCHUS-MYKISS ; EARLY-LIFE STAGES ; SALMO-SALAR L. ; BACTERIAL COMMUNITIES ; MOLECULAR CHARACTERIZATION ; PHYLOGENETIC ANALYSIS ; ZEBRAFISH GUT ; WATER FISH
收录类别	SCI
资助信息	Major State Basic Research Development Program of China[2009CB118705]; National Natural Science Foundation of China[31172084]
语种	英语
WOS记录号	WOS:000301013300004
公开日期	2012-04-01
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16705]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, Xuemei,Yu, Yuhe,Feng, Weisong,et al. Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae[J]. JOURNAL OF MICROBIOLOGY,2012,50(1):29-37.
APA	Li, Xuemei,Yu, Yuhe,Feng, Weisong,Yan, Qingyun,&Gong, Yingchun.(2012).Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae.JOURNAL OF MICROBIOLOGY,50(1),29-37.
MLA	Li, Xuemei,et al."Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae".JOURNAL OF MICROBIOLOGY 50.1(2012):29-37.