The response of carbohydrate metabolism to the fluctuation of relative humidity (RH) in the desert soil cyanobacterium Phormidium tenue

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	The response of carbohydrate metabolism to the fluctuation of relative humidity (RH) in the desert soil cyanobacterium Phormidium tenue
	Chen, Lanzhou 1; Yang, Yan 1; Deng, Songdiang 1; Xu, Yanhong 1; Wang, Gaohong 2; Liu, Yongding 2
刊名	EUROPEAN JOURNAL OF SOIL BIOLOGY
	2012
卷号	48 页码:11-16
关键词	Biological soil crusts Exoplysaccharides (EPS) Desiccation Oxidative damage
ISSN号	1164-5563
通讯作者	Chen, LZ (reprint author), Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China
中文摘要	The excreting of exopolysaccharides (EPS) is thought as one of the main protection ways for cyanobacteria in desert algal crusts to survive desiccation. But how cyanobacteria adjust their carbohydrate metabolism to survive this stress is not elucidated. In this study, we treated Phormidium tenue, a cyanobacterium isolated from biological soil crusts with the changes of relative humidity (RH) to simulate different levels of desiccation and investigated its carbohydrate metabolism. It was found that photosynthetic activity (Fv/Fm) and cellular total carbohydrates production decreased significantly at low RH. But the production of EPS, reducing sugar, sucrose and the activity of sucrose phosphate synthase (SPS) increased significantly at low RH and reached maximum at 75% RH. Low RH could also cause the enhanced production of reactive oxygen species (ROS) generation, malondialdehyde (MDA) production and DNA strand breaks. However, when pretreated with exogenous 100 mg/L EPS, Fv/Fm and carbohydrate production were improved significantly, while ROS generation, MDA production and DNA strand breaks decreased significantly at various levels of RH in P tenue. These results indicated that P. tenue in arid regions could enhance desiccation tolerance by adjusting the carbohydrate metabolism to eliminate ROS and decrease oxidative damage. (C) 2011 Elsevier Masson SAS. All rights reserved.
英文摘要	The excreting of exopolysaccharides (EPS) is thought as one of the main protection ways for cyanobacteria in desert algal crusts to survive desiccation. But how cyanobacteria adjust their carbohydrate metabolism to survive this stress is not elucidated. In this study, we treated Phormidium tenue, a cyanobacterium isolated from biological soil crusts with the changes of relative humidity (RH) to simulate different levels of desiccation and investigated its carbohydrate metabolism. It was found that photosynthetic activity (Fv/Fm) and cellular total carbohydrates production decreased significantly at low RH. But the production of EPS, reducing sugar, sucrose and the activity of sucrose phosphate synthase (SPS) increased significantly at low RH and reached maximum at 75% RH. Low RH could also cause the enhanced production of reactive oxygen species (ROS) generation, malondialdehyde (MDA) production and DNA strand breaks. However, when pretreated with exogenous 100 mg/L EPS, Fv/Fm and carbohydrate production were improved significantly, while ROS generation, MDA production and DNA strand breaks decreased significantly at various levels of RH in P tenue. These results indicated that P. tenue in arid regions could enhance desiccation tolerance by adjusting the carbohydrate metabolism to eliminate ROS and decrease oxidative damage. (C) 2011 Elsevier Masson SAS. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Ecology ; Soil Science
研究领域[WOS]	Environmental Sciences & Ecology ; Agriculture
关键词[WOS]	DESICCATION TOLERANCE ; MICROCOLEUS-VAGINATUS ; OXIDATIVE DAMAGE ; SALT STRESS ; DNA-DAMAGE ; EXOPOLYSACCHARIDES ; CRUSTS ; POLYSACCHARIDES ; PHOTOSYNTHESIS ; INVOLVEMENT
收录类别	SCI
资助信息	foundation of Plan of Wuhan Scientific Chenguang of Young People[200850731367]; National Natural Science Foundation of China[30970446, 30500068, 30970688
语种	英语
WOS记录号	WOS:000300036500002
公开日期	2012-04-01
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16757]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Chen, Lanzhou,Yang, Yan,Deng, Songdiang,et al. The response of carbohydrate metabolism to the fluctuation of relative humidity (RH) in the desert soil cyanobacterium Phormidium tenue[J]. EUROPEAN JOURNAL OF SOIL BIOLOGY,2012,48:11-16.
APA	Chen, Lanzhou,Yang, Yan,Deng, Songdiang,Xu, Yanhong,Wang, Gaohong,&Liu, Yongding.(2012).The response of carbohydrate metabolism to the fluctuation of relative humidity (RH) in the desert soil cyanobacterium Phormidium tenue.EUROPEAN JOURNAL OF SOIL BIOLOGY,48,11-16.
MLA	Chen, Lanzhou,et al."The response of carbohydrate metabolism to the fluctuation of relative humidity (RH) in the desert soil cyanobacterium Phormidium tenue".EUROPEAN JOURNAL OF SOIL BIOLOGY 48(2012):11-16.