Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry

	Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry
	Liu L(刘丽); Jin G(靳刚); Niu Y(牛宇)
	2011
会议名称	5th International Conference on Spectroscopic Ellipsometry
会议日期	MAY 23-29, 2010
通讯作者邮箱	gajin@imech.ac.cn
会议地点	Albany, NY
关键词	Ricin detection Antigen-antibody interaction Biosensor Imaging ellipsometry
卷号	519
期号	9
页码	2768-2771
通讯作者	Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
中文摘要	In order to meet the requirements of antibody comparison and evaluation, the optimization for the surface modification, ligand immobilization, unspecific blocking and ligand receptor interaction condition is introduced to the biosensor based on imaging ellipsometry (BIE), so that it may serve as an antibody screening platform to evaluate antibody antigen interactions. Two kinds of anti-ricin antibody named pVHHS1 and 5S1R are immobilized in a patterned array format on the silicon surface modified with succinic anhydride, respectively, to form a sensing surface. Ricin and its similar toxin abrin as the reference in solution are delivered, respectively, on the patterned sensing surface for anti-ricin antibody and ricin interaction. The limit of detection value of ricin detected by pVHHS1 and 5S1R is obtained as 1 mu g/ml and 5 ng/ml, respectively. Results show that pVHHS1 and 5S1R specifically bind with ricin but not with abrin, which is in accordance with ELISA's result. (C) 2010 Elsevier B.V. All rights reserved.
收录类别	CPCI（ISTP）
合作状况	国内
产权排序	Chinese Acad Sci, NML, Inst Mech, Beijing 100190, Peoples R China; Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
会议主办者	Univ Albany, Coll Nanoscale & Engn
会议网址	http://dx.doi.org/10.1016/j.tsf.2010.12.054
会议录	THIN SOLID FILMS
会议录出版者	ELSEVIER SCIENCE SA
会议录出版地	PO BOX 564, 1001 LAUSANNE, SWITZERLAND
学科主题	Materials Science, Multidisciplinary; Materials Science, Coatings & Films; Physics, Applied; Physics, Condensed Matter
语种	英语
ISSN号	0040-6090
内容类型	会议论文
源URL	[http://dspace.imech.ac.cn/handle/311007/45328]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Liu L,Jin G,Niu Y. Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry[C]. 见:5th International Conference on Spectroscopic Ellipsometry. Albany, NY. MAY 23-29, 2010.http://dx.doi.org/10.1016/j.tsf.2010.12.054.