Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow

	Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow
	Fu ZL(傅长亮); Tong CF(佟春芳); Dong C; Long M(龙勉)
刊名	Cellular and Molecular Bioengineering
	2011
通讯作者邮箱	mlong@imech.ac.cn
卷号	4 期号:3 页码:427-441
关键词	Two-Dimensional Kinetics Cone-Plate Viscometer Homotypic Aggregation Heterotypic Aggregation Bell Model Protein G-Igg Bond Beta(2)-Integrin And Icam-1 Bond Human Blood-Platelets Intercellular-Adhesion Molecule-1 Hydrodynamic Shear Disaggregation Kinetics Neutrophil Aggregation Sequential Binding Mediated Adhesion Stable Adhesion Melanoma-Cells Latex Spheres
ISSN号	1865-5025
通讯作者	Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China
产权排序	[Fu, Changliang; Tong, Chunfang; Long, Mian] Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China; [Fu, Changliang; Tong, Chunfang; Long, Mian] Chinese Acad Sci, Natl Micrograv Lab, Inst Mech, Beijing 100190, Peoples R China; [Fu, Changliang; Tong, Chunfang; Long, Mian] Chinese Acad Sci, Ctr Biomech & Bioengn, Inst Mech, Beijing 100190, Peoples R China; [Dong, Cheng] Penn State Univ, Dept Bioengn, University Pk, PA 16802 USA
合作状况	国际
中文摘要	Shear-induced cell aggregation and disaggregation, governed by specific receptor-ligand binding, play important roles in many biological and biophysical processes. While a lot of studies have focused on elucidating the shear rate and shear stress dependence of cell aggregation, the majority of existing models based on population balance equation (PBE) has rarely dealt with cell aggregation dynamics upon intrinsic molecular kinetics. Here, a kinetic model was developed for further understanding cell aggregation and disaggregation in a linear shear flow. The novelty of the model is that a set of simple equations was constructed by coupling two-body collision theory with receptor-ligand binding kinetics. Two cases of study were employed to validate the model: one is for the homotypic aggregation dynamics of latex beads cross-linked by protein G-IgG binding, and the other is for the heterotypic aggregation dynamics of neutrophils-tumor cells governed by beta 2-integrin-ligand interactions. It was found that the model fits the data well and the obtained kinetic parameters are consistent with the previous predictions and experimental measurements. Moreover, the decay factor defined biophysically to account for the chemokine- and shear-induced regulation of receptor and/or ligand expression and conformation was compared at molecular and cellular levels. Our results provided a universal framework to quantify the molecular kinetics of receptor-ligand binding in shear-induced cell aggregation dynamics.
学科主题	Cell Biology; Biophysics
分类号	Q4
类目[WOS]	Cell & Tissue Engineering ; Biophysics ; Cell Biology
研究领域[WOS]	Cell Biology ; Biophysics
关键词[WOS]	HUMAN BLOOD-PLATELETS ; INTERCELLULAR-ADHESION MOLECULE-1 ; HYDRODYNAMIC SHEAR ; DISAGGREGATION KINETICS ; NEUTROPHIL AGGREGATION ; SEQUENTIAL BINDING ; MEDIATED ADHESION ; STABLE ADHESION ; MELANOMA-CELLS ; LATEX SPHERES
收录类别	SCI
资助信息	This work was supported by National Natural Science Foundation of China grants 30730032, 11072251, 10902117, and 10702075, Chinese Academy of Sciences grants KJCX2-YW-L08 and Y2010030, and National Key Basic Research Foundation of China grant 2011CB710904.
原文出处	http://dx.doi.org/10.1007/s12195-011-0167-x
语种	英语
WOS记录号	WOS:000297866300011
公开日期	2012-04-01
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/45075]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Fu ZL,Tong CF,Dong C,et al. Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow[J]. Cellular and Molecular Bioengineering,2011,4(3):427-441.
APA	傅长亮,佟春芳,Dong C,&龙勉.(2011).Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow.Cellular and Molecular Bioengineering,4(3),427-441.
MLA	傅长亮,et al."Modeling of Cell Aggregation Dynamics Governed by Receptor-Ligand Binding Under Shear Flow".Cellular and Molecular Bioengineering 4.3(2011):427-441.