Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay

	Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay
	Fu ZL(傅长亮); Tong CF(佟春芳); Wang ML; Gao YX(高宇欣); Zhang Y(章燕); Lv SQ(吕守芹); Liang SL; Dong C; Long M(龙勉)
刊名	Journal of Biological Chemistry
	2011
通讯作者邮箱	mlong@imech.ac.cn
卷号	286 期号:40 页码:34777-34787
关键词	Mammary Adenocarcinoma Cells Hydrodynamic Shear Melanoma Adhesion 2-Dimensional Kinetics Human Neutrophils P-Selectin Metastasis Development Ligand Interactions Concurrent Binding Cancer Metastasis
ISSN号	0021-9258
通讯作者	Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
产权排序	[Fu, Changliang; Tong, Chunfang; Wang, Manliu; Gao, Yuxin; Zhang, Yan; Lu, Shouqin; Long, Mian] Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China; [Fu, Changliang; Tong, Chunfang; Wang, Manliu; Gao, Yuxin; Zhang, Yan; Lu, Shouqin; Long, Mian] Chinese Acad Sci, Natl Micrograv Lab, Beijing 100190, Peoples R China; [Fu, Changliang; Tong, Chunfang; Wang, Manliu; Gao, Yuxin; Zhang, Yan; Lu, Shouqin; Long, Mian] Chinese Acad Sci, Ctr Biomech & Bioengn, Inst Mech, Beijing 100190, Peoples R China; [Liang, Shile; Dong, Cheng] Penn State Univ, Dept Bioengn, University Pk, PA 16802 USA
合作状况	国际
中文摘要	Interactions between polymorphonuclear neutrophils (PMNs) and tumor cells have been reported to facilitate the adhesion and subsequent extravasation of tumor cells through the endothelium under blood flow, both of which are mediated by binding beta(2)-integrin to intercellular adhesion molecule 1 (ICAM-1). Here the adhesions between humanWM9 metastatic melanoma cells, PMNs, and human pulmonary microvascular endothelial cells (HPMECs) were quantified by a gas-driven micropipette aspiration technique (GDMAT). Our data indicated that the cellular binding affinity of PMN-WM9 pair was 3.9-fold higher than that of the PMN-HPMEC pair. However, the effective binding affinities per molecular pair were comparable between the two cell pairs no matter whether WM9 cells or HPMECs were quiescent or cytokine-activated, indicating that the stronger adhesion between PMN-WM9 pair is mainly attributed to the high expression of ICAM-1 on WM9 cells. These results proposed an alternative mechanism, where WM9 melanoma cells adhere first with PMNs near vessel-wall regions and then bind to endothelial cells via PMNs under blood flow. In contrast, the adhesions between human MDA-MB-231 metastatic breast carcinoma cells and PMNs showed a comparable cellular binding affinity to PMN-HPMEC pair because the ICAM-1 expressions on MDA-MB-231 cells and HPMECs are similar. Furthermore, differences were observed in the intrinsic forward and reverse rates of the beta(2)-integrin-ICAM-1 bond between PMN-TC and PMN-EC pairs. This GDMAT assay enables us to quantify the binding kinetics of cell adhesion molecules physiologically expressed on nucleated cells. The findings also further the understanding of leukocyte-facilitated tumor cell adhesion from the viewpoint of molecular binding kinetics.
学科主题	Biochemistry & Molecular Biology
分类号	二类/Q2
类目[WOS]	Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	MAMMARY ADENOCARCINOMA CELLS ; HYDRODYNAMIC SHEAR ; MELANOMA ADHESION ; 2-DIMENSIONAL KINETICS ; HUMAN NEUTROPHILS ; P-SELECTIN ; METASTASIS DEVELOPMENT ; LIGAND INTERACTIONS ; CONCURRENT BINDING ; CANCER METASTASIS
收录类别	SCI
资助信息	This work was supported, in whole or in part, by National Institutes of Health Grants CA-97306, CA-125707, M01-RR-010732, and C06-RR-016499. This work was also supported by National Natural Science Foundation of China Grants 30730032, 10702075, and 10902117; National Key Basic Research Foundation of China Grants 2006CB910303 and 2011CB710904; Strategic Priority Research Program of Chinese Academy of Science grants XDA01030102 and XDA04020219, Knowledge Innovation Program of Chinese Academy of Science Grant KJCX2-YW-L08 (to M.L.), and National Science Foundation Grants BES-0138474 and CBET-0729091 (to C.D.).
原文出处	http://dx.doi.org/10.1074/jbc.M111.281642
语种	英语
WOS记录号	WOS:000295406300032
公开日期	2012-04-01
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/45069]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Fu ZL,Tong CF,Wang ML,et al. Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay[J]. Journal of Biological Chemistry,2011,286(40):34777-34787.
APA	傅长亮.,佟春芳.,Wang ML.,高宇欣.,章燕.,...&龙勉.(2011).Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay.Journal of Biological Chemistry,286(40),34777-34787.
MLA	傅长亮,et al."Determining beta(2)-Integrin and Intercellular Adhesion Molecule 1 Binding Kinetics in Tumor Cell Adhesion to Leukocytes and Endothelial Cells by a Gas-driven Micropipette Assay".Journal of Biological Chemistry 286.40(2011):34777-34787.