DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis

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	DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis
	Liu, W ; Li, PJ ; Qi, XM ; Zhou, Q ; Zheng, L ; Sun, TH ; Yang, YS
刊名	CHEMOSPHERE
	2005
卷号	61 期号:2 页码:158-167
关键词	cadmium (Cd) root growth total soluble protein content DNA polymorphism change genomic template stability biomarker
ISSN号	0045-6535
通讯作者	Liu, W, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
中文摘要	In recent years, several plant species have been used as bioindicators, and several tests have been developed to evaluate the toxicity of environmental contaminants on vegetal organisms. In this study, barley (Hordeum vulgare L) seedling was used as bioindicator of cadmium (Cd) pollution in the range of 30-120 mg l(-1). Inhibition of root growth and reduction of total soluble protein content in root tips of barley seedlings were observed with the increase of Cd concentrations. The changes occurring in random amplified polymorphic DNA (RAPD) profiles of root tips following Cd treatment included variation in band intensity, loss of normal bands and appearance of new bands compared with the normal seedlings. Additionally, we found that the effect of changes was dose-dependent. These results indicated that genomic template stability (a qualitative measure reflecting changes in RAPD profiles) was significantly affected at the above Cd concentration. Thus, DNA polymorphisms detected by RAPD analysis could be used as an investigation tool for environmental toxicology and as a useful biomarker assay for the detection of genotoxic effects of Cd pollution on plants. (C) 2005 Elsevier Ltd. All rights reserved.
学科主题	Environmental Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000232498700002
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/55063]
专题	沈阳应用生态研究所_沈阳应用生态研究所_期刊论文
推荐引用方式 GB/T 7714	Liu, W,Li, PJ,Qi, XM,et al. DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis[J]. CHEMOSPHERE,2005,61(2):158-167.
APA	Liu, W.,Li, PJ.,Qi, XM.,Zhou, Q.,Zheng, L.,...&Yang, YS.(2005).DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis.CHEMOSPHERE,61(2),158-167.
MLA	Liu, W,et al."DNA changes in barley (Hordeum vulgare) seedlings induced by cadmium pollution using RAPD analysis".CHEMOSPHERE 61.2(2005):158-167.