Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus

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	Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus
	Jiang, Wenbo 1; Yang, Yuhui 1; Zhao, Dongmei 1; Liu, Xiaoyan 1; Duan, Jundan 1; Xie, Songguang 2; Zhao, Haobin 1
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
	2011-12-01
卷号	160 期号:4 页码:187-193
关键词	Gobiocypris rarus Sex determination and sex differentiation foxl2 foxl2b Expression pattern
ISSN号	1096-4959
通讯作者	Zhao, HB (reprint author), Huazhong Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrated Biol, Wuhan 430079, Hubei, Peoples R China ; zhaohb@mail.ccnu.edu.cn
中文摘要	Gobiocypris rams is an emerging fish model for aquatic toxicology in China as it is sensitive to environmental hormone disruptors. Exogenous sex steroids can affect sex differentiation and the expression of sex-related genes. Foxl2, a member of forkhead-box transcription factor family, is the key gene for ovary development and its mutation causes the blepharophimosis ptosis epicanthus inversus syndrome in human. We find that two foxl2 genes exist in fish genome, one is foxl2, and the other is foxl2b. Here, we reported the isolation and expression of foxl2 in G. rams. G. rarus foxl2 cDNA is 1700 bp in length with a 921 bp of open reading frame encoding 306 amino acids containing the typical FM-domain. Semi-quantitative RT-PCR revealed its predominant expression in the eye, brain, gill and gonads. Moreover, the expression level in the ovary was significantly higher than that in the testis. Quantitative RT-PCR showed that foxl2 was up regulated after treatment with estradiol and was down regulated with 2-methyl-testosterone. These results suggested that Foxl2 plays an important role in female development of G. rams, foxl2 mRNA expression is regulated by downstream sex hormones, and foxl2 can be used as a molecular indicator monitoring the environmental endocrine disruptors. (C) 2011 Elsevier Inc. All rights reserved.
英文摘要	Gobiocypris rams is an emerging fish model for aquatic toxicology in China as it is sensitive to environmental hormone disruptors. Exogenous sex steroids can affect sex differentiation and the expression of sex-related genes. Foxl2, a member of forkhead-box transcription factor family, is the key gene for ovary development and its mutation causes the blepharophimosis ptosis epicanthus inversus syndrome in human. We find that two foxl2 genes exist in fish genome, one is foxl2, and the other is foxl2b. Here, we reported the isolation and expression of foxl2 in G. rams. G. rarus foxl2 cDNA is 1700 bp in length with a 921 bp of open reading frame encoding 306 amino acids containing the typical FM-domain. Semi-quantitative RT-PCR revealed its predominant expression in the eye, brain, gill and gonads. Moreover, the expression level in the ovary was significantly higher than that in the testis. Quantitative RT-PCR showed that foxl2 was up regulated after treatment with estradiol and was down regulated with 2-methyl-testosterone. These results suggested that Foxl2 plays an important role in female development of G. rams, foxl2 mRNA expression is regulated by downstream sex hormones, and foxl2 can be used as a molecular indicator monitoring the environmental endocrine disruptors. (C) 2011 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Zoology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Zoology
研究领域[WOS]	Biochemistry & Molecular Biology ; Zoology
关键词[WOS]	TRANSCRIPTION FACTOR FOXL2 ; MOLECULAR-CLONING ; GENE-EXPRESSION ; OVARIAN DEVELOPMENT ; OREOCHROMIS-NILOTICUS ; INVERSUS SYNDROME ; ORYZIAS-LATIPES ; NILE TILAPIA ; DIFFERENTIATION ; AROMATASE
收录类别	SCI
资助信息	National Key Laboratory of Aquatic Diversity and Resource Conservation; Hubei Key Laboratory of Genetic Regulation and Integrative Biology
语种	英语
WOS记录号	WOS:000296679800007
公开日期	2011-12-22
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16411]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	1.Huazhong Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrated Biol, Wuhan 430079, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Natl Key Lab Aquat Biodivers & Resource Conservat, Wuhan 430072, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Wenbo,Yang, Yuhui,Zhao, Dongmei,et al. Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2011,160(4):187-193.
APA	Jiang, Wenbo.,Yang, Yuhui.,Zhao, Dongmei.,Liu, Xiaoyan.,Duan, Jundan.,...&Zhao, Haobin.(2011).Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,160(4),187-193.
MLA	Jiang, Wenbo,et al."Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 160.4(2011):187-193.