Hormesis of mercuric chloride-human serum albumin adduct on N9 microglial cells via the ERK/MAPKs and JAK/STAT3 signaling pathways

	Hormesis of mercuric chloride-human serum albumin adduct on N9 microglial cells via the ERK/MAPKs and JAK/STAT3 signaling pathways
	Tan, Qiaozhu; Liu, Zhitao; Li, Hong; Liu, Yongjun; Xia, Zhenghua; Xiao, Yuancan; Usman, Muhammad; Du, Yuzhi; Bi, Hongtao; Wei, Lixin
刊名	TOXICOLOGY
	2018
卷号	408
关键词	Mercuric chloride Hg (II)-serum albumin adduct N9 microglial cell Hormesis Hg2+-binding site Coordination structure
英文摘要	Mercury chloride (HgCl2), a neurotoxicant that cannot penetrate the blood-brain barrier (BBB). Although when the BBB are got damaged by neurodegenerative disorders, the absorbed HgCl2, mainly in form of Hg (ID-serum albumin adduct (Hg-HSA) in human plasma, can penetrate BBB and affect central nervous system (CNS) cells. Current study planned to evaluate the effect of Hg-HSA on the physiological function of N9 microglial cells. At low dosage (15 ng/mL) of Hg-HAS, the observed outcomes was: promoted cell propagation, Nitric Oxide (NO) and intracellular Ca2+ levels enhancement, suppressed the release of TNF-alpha and IL-1 beta and inhibited cell proliferation. At high dosage (15 mu g/mL) we observed decline in NO and intracellular Ca2+ levels, and increment in the release of TNF-alpha and IL-1 beta. These biphasic effects are similar to hormesis, and the hormesis, in this case, was executed through ERK/MAPKs and JAK/STAT3 signaling pathways. Study of quantum chemistry revealed that Hg2+ could form stable coordination structures in both Asp249 and Cys34 sites of HSA. Although five-co-ordination structure in Asp249 site is more stable than four-coordination structure in Cys34 site but four-co-ordination structure is formed easily in-vivo in consideration of binding-site position in spatial structure of HSA.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/59988]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Tan, Qiaozhu,Liu, Zhitao,Li, Hong,et al. Hormesis of mercuric chloride-human serum albumin adduct on N9 microglial cells via the ERK/MAPKs and JAK/STAT3 signaling pathways[J]. TOXICOLOGY,2018,408.
APA	Tan, Qiaozhu.,Liu, Zhitao.,Li, Hong.,Liu, Yongjun.,Xia, Zhenghua.,...&Wei, Lixin.(2018).Hormesis of mercuric chloride-human serum albumin adduct on N9 microglial cells via the ERK/MAPKs and JAK/STAT3 signaling pathways.TOXICOLOGY,408.
MLA	Tan, Qiaozhu,et al."Hormesis of mercuric chloride-human serum albumin adduct on N9 microglial cells via the ERK/MAPKs and JAK/STAT3 signaling pathways".TOXICOLOGY 408(2018).