A ratiometric detection of heparin with high sensitivity based on aggregation-enhanced emission of gold nanoclusters triggered by silicon nanoparticles

	A ratiometric detection of heparin with high sensitivity based on aggregation-enhanced emission of gold nanoclusters triggered by silicon nanoparticles
	Qu, Fei; Xia, Wenle; Xia, Lian; You, Jinmao; Han, Wenli
刊名	TALANTA
	2019
卷号	193
关键词	Self-assembly Aggregation-enhanced emission Ratiometric fluorescence Silicon nanoparticles Gold nanoclusters Heparin
英文摘要	Heparin (Hep) is a widely applied anticoagulant and the quantification of heparin concentration is pivotal for clinical use. In this work, silicon nanoparticles (SiNPs) modified by the amino groups and glutathione-capped gold nanoclusters (GSH-AuNCs) are able to self-assemble into spherical particle structures via the electrostatic interaction, resulting in the aggregation-enhanced emission (AEE) of GSH-AuNCs. However, Hep, a highly sulfated glycosaminoglycan with much more negative charges, can bind with the SiNPs and inhibit the aggregation. As a result, it causes the AEE quenching of GSH-AuNCs at 570 nm but the SiNPs keep their own blue fluorescence at 450 nm. Thus, the SiNPs can act as an internal reference and the GSH-AuNCs are used as a signal probe in this process. The ratiometric fluorescent signal (I-570/I-450) change of the nanohybrid probe is positively correlated with Hep concentrations in the range from 6.44 ng/mL to 96.6 ng/mL with the detection limit of 3.29 ng/mL. As expected, this strategy shows good sensitivity and selectivity, and it is also successfully applied to detect Hep in Hep sodium injection and human serum samples with good recoveries.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/59871]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Qu, Fei,Xia, Wenle,Xia, Lian,et al. A ratiometric detection of heparin with high sensitivity based on aggregation-enhanced emission of gold nanoclusters triggered by silicon nanoparticles[J]. TALANTA,2019,193.
APA	Qu, Fei,Xia, Wenle,Xia, Lian,You, Jinmao,&Han, Wenli.(2019).A ratiometric detection of heparin with high sensitivity based on aggregation-enhanced emission of gold nanoclusters triggered by silicon nanoparticles.TALANTA,193.
MLA	Qu, Fei,et al."A ratiometric detection of heparin with high sensitivity based on aggregation-enhanced emission of gold nanoclusters triggered by silicon nanoparticles".TALANTA 193(2019).