Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer
	Li Jialin1,2 ; Bai Jie 1; Gao Huiwang 1; Liu Guangxing 1
刊名	ACTA OCEANOLOGICA SINICA
	2011-05-01
卷号	30 期号:3 页码:92-99
关键词	ammonia-oxidizing Betaproteobacteria (beta AOB) diversity abundance nitrification surface sediment Changjiang River Estuary (CRE)
ISSN号	0253-505X
通讯作者	Ocean Univ China, Coll Environm Sci & Engn, Minist Educ, Key Lab Marine Environm Sci & Ecol, Qingdao 266100, Peoples R China. baijie@ouc.edu.cn
产权排序	[Li, JL; Bai, J; Gao, HW; Liu, GX] Ocean Univ China, Coll Environm Sci & Engn, Minist Educ, Key Lab Marine Environm Sci & Ecol, Qingdao 266100, Peoples R China; [Li, JL] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
中文摘要	The spatial distribution of ammonia-oxidizing Betaproteobacteria (beta AOB) was investigated by FISH (fluorescence in situ hybridization) and DGGE (denaturing gradient gel electrophoresis) techniques in the sediment off the Changjiang River Estuary. Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006. The abundance of beta AOB ranged from 1.87 x 10(5) to 3.53 x 10(5) cells/g of sediment. beta AOB abundance did not present a negative correlation with salinity, whereas salinity was implicated as the primary factor affecting nitrification rates. The DGGE profiles of PCR-amplified amoA gene fragments revealed that the beta AOB community structure of sample S2 separated from other samples at the level of 40% similarity. The variations in composition of beta AOB were significantly correlated with the salinity, temperature, absorption ability of sediments and TOC. The statistical analysis indicates that the beta AOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity. Considering the good correlation between beta AOB abundance and nitrification estimates, the abundance and diversity of beta AOB community could be expected as an indirect index of nitrification activity at the study sea area in summer.
英文摘要	The spatial distribution of ammonia-oxidizing Betaproteobacteria (beta AOB) was investigated by FISH (fluorescence in situ hybridization) and DGGE (denaturing gradient gel electrophoresis) techniques in the sediment off the Changjiang River Estuary. Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006. The abundance of beta AOB ranged from 1.87 x 10(5) to 3.53 x 10(5) cells/g of sediment. beta AOB abundance did not present a negative correlation with salinity, whereas salinity was implicated as the primary factor affecting nitrification rates. The DGGE profiles of PCR-amplified amoA gene fragments revealed that the beta AOB community structure of sample S2 separated from other samples at the level of 40% similarity. The variations in composition of beta AOB were significantly correlated with the salinity, temperature, absorption ability of sediments and TOC. The statistical analysis indicates that the beta AOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity. Considering the good correlation between beta AOB abundance and nitrification estimates, the abundance and diversity of beta AOB community could be expected as an indirect index of nitrification activity at the study sea area in summer.
学科主题	Oceanography
研究领域[WOS]	Oceanography
关键词[WOS]	EAST CHINA SEA ; 16S RIBOSOMAL-RNA ; ENVIRONMENTAL GRADIENTS ; SPATIAL-DISTRIBUTION ; NITRIFICATION RATES ; BACTERIA ; DIVERSITY ; ARCHAEA ; NITROGEN ; OCEAN
收录类别	SCI
资助信息	National Fundamental Project of China[2006CB400602]
语种	英语
WOS记录号	WOS:000292673000011
公开日期	2011-11-11
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5116]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Ocean Univ China, Coll Environm Sci & Engn, Minist Educ, Key Lab Marine Environm Sci & Ecol, Qingdao 266100, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Li Jialin,Bai Jie,Gao Huiwang,et al. Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer[J]. ACTA OCEANOLOGICA SINICA,2011,30(3):92-99.
APA	Li Jialin,Bai Jie,Gao Huiwang,&Liu Guangxing.(2011).Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer.ACTA OCEANOLOGICA SINICA,30(3),92-99.
MLA	Li Jialin,et al."Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer".ACTA OCEANOLOGICA SINICA 30.3(2011):92-99.