Identification and sexually dimorphic expression of vasa isoforms in Dabry's sturgeon (Acipenser dabryanus), and functional analysis of vasa 3'-untranslated region | |
Ye, Huan1,2; Yue, Hua-Mei2,3; Yang, Xiao-Ge1,2; Li, Chuang-Ju2,3; Wei, Qi-1,2,3 | |
刊名 | CELL AND TISSUE RESEARCH |
2016-10-01 | |
卷号 | 366期号:1页码:203-218 |
关键词 | Dabry's sturgeon Vasa Dimorphic expression Primordial germ cells Visualization |
ISSN号 | 0302-766X |
DOI | 10.1007/s00441-016-2418-6 |
通讯作者 | Li, Chuang-Ju(lcj@yfi.ac.cn) ; Wei, Qi-(weiqw@yfi.ac.cn) |
英文摘要 | Germ cells are set aside from somatic cells early in embryogenesis, and are responsible for transmitting genetic information through generations. Vasa is a highly conserved germ cell marker across animal phyla, and widely used to label primordial germ cells. Dabry's sturgeon is a rare and endangered species distributed solely in the Yangtze River basin. Here, seven vasa isoforms, named Advasa1-7, were isolated and characterized in Dabry's sturgeon. RT-PCR and western blot analyses revealed that vasa mRNA and protein were mainly restricted to the testis and ovary, but exhibited sexually dimorphic expression. Cellular and subcellular localization uncovered that Advasa mRNA and protein displayed mitotic and meiotic expression in females, and mainly showed mitotic expression in males; surprisingly, they exhibited both cytoplasmic and nuclear expression in the ovarian germ cells, while showing exclusively cytoplasmic expression in the testicular germ cells. By microinjecting chimeric RNA consisting of the red fluorescent protein coding region and the Advasa 3'-untranslated region into embryos of Dabry's sturgeon, zebrafish and medaka, we demonstrated that it had the ability to visualize primordial germ cells (PGCs) in Dabry's sturgeon and zebrafish but not in medaka. It seemed that the machinery of vasa 3'UTR RNA localization was conserved between Dabry's sturgeon and ostariophysan, while possibly changed during the divergence of euteleosts and ostariophysan. Finally, Dabry's sturgeon PGCs moved on the yolk ball, and migrated toward the genital ridge via mesenchyme. Taken together, these results provide new information for vasa expression pattern and function, and lay a foundation for PGC cryopreservation and conservation of Dabry's sturgeon. |
资助项目 | National Natural Science Foundation of China[31472286] ; National Natural Science Foundation of China[31172413] ; National Key Basic Research Program of China[2015CB150702] ; Special Fund for Agro-Scientific Research in the Public Interest[201203086] |
WOS关键词 | PRIMORDIAL GERM-CELLS ; DIFFERENTIAL EXPRESSION ; ZEBRAFISH VASA ; HOMOLOG GENE ; MOLECULAR CHARACTERIZATION ; DROSOPHILA-VASA ; YANGTZE-RIVER ; TELEOST FISH ; PROTEIN ; RNA |
WOS研究方向 | Cell Biology |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000385139100017 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Basic Research Program of China ; National Key Basic Research Program of China ; National Key Basic Research Program of China ; National Key Basic Research Program of China ; Special Fund for Agro-Scientific Research in the Public Interest ; Special Fund for Agro-Scientific Research in the Public Interest ; Special Fund for Agro-Scientific Research in the Public Interest ; Special Fund for Agro-Scientific Research in the Public Interest ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Basic Research Program of China ; National Key Basic Research Program of China ; National Key Basic Research Program of China ; National Key Basic Research Program of China ; Special Fund for Agro-Scientific Research in the Public Interest ; Special Fund for Agro-Scientific Research in the Public Interest ; Special Fund for Agro-Scientific Research in the Public Interest ; Special Fund for Agro-Scientific Research in the Public Interest ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Basic Research Program of China ; National Key Basic Research Program of China ; National Key Basic Research Program of China ; National Key Basic Research Program of China ; Special Fund for Agro-Scientific Research in the Public Interest ; Special Fund for Agro-Scientific Research in the Public Interest ; Special Fund for Agro-Scientific Research in the Public Interest ; Special Fund for Agro-Scientific Research in the Public Interest ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key Basic Research Program of China ; National Key Basic Research Program of China ; National Key Basic Research Program of China ; National Key Basic Research Program of China ; Special Fund for Agro-Scientific Research in the Public Interest ; Special Fund for Agro-Scientific Research in the Public Interest ; Special Fund for Agro-Scientific Research in the Public Interest ; Special Fund for Agro-Scientific Research in the Public Interest |
内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/34140] |
专题 | 水生生物研究所_其他_期刊论文 |
通讯作者 | Li, Chuang-Ju; Wei, Qi- |
作者单位 | 1.Univ Chinese Acad Sci, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China 2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Wuhan 430223, Peoples R China 3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China |
推荐引用方式 GB/T 7714 | Ye, Huan,Yue, Hua-Mei,Yang, Xiao-Ge,et al. Identification and sexually dimorphic expression of vasa isoforms in Dabry's sturgeon (Acipenser dabryanus), and functional analysis of vasa 3'-untranslated region[J]. CELL AND TISSUE RESEARCH,2016,366(1):203-218. |
APA | Ye, Huan,Yue, Hua-Mei,Yang, Xiao-Ge,Li, Chuang-Ju,&Wei, Qi-.(2016).Identification and sexually dimorphic expression of vasa isoforms in Dabry's sturgeon (Acipenser dabryanus), and functional analysis of vasa 3'-untranslated region.CELL AND TISSUE RESEARCH,366(1),203-218. |
MLA | Ye, Huan,et al."Identification and sexually dimorphic expression of vasa isoforms in Dabry's sturgeon (Acipenser dabryanus), and functional analysis of vasa 3'-untranslated region".CELL AND TISSUE RESEARCH 366.1(2016):203-218. |
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