Development of an image biosensor based on an optogenetically engineered cell for visual prostheses

CORC > 沈阳自动化研究所 > 中国科学院沈阳自动化研究所 > 机器人学研究室

	Development of an image biosensor based on an optogenetically engineered cell for visual prostheses
	Yang WG(杨文广)2; Li GX(李恭新)4,5,6; Wang FF(王飞飞)3; Liu LQ(刘连庆)5,6 ; Wang WX(王文学)5,6 ; Wang YC(王越超)5,6 ; Yang J(杨佳)1,5,6
刊名	Nanoscale
	2019
卷号	11 期号:28 页码:13213-13218
ISSN号	2040-3364
产权排序	1
英文摘要	Visual prostheses provide blind patients with artificial vision via electrical stimulation of surviving visual cells resulting in partial restoration of vision in many patients. However, high-resolution visual perception, long-term biocompatibility and safety remain the significant challenges of existing visual prostheses. Here, we present a novel method to develop a new visual prosthesis using living cells as integrated electronics and implantable microelectrodes. The living cells modified with channelrhodopsin-2 showed excellent light-sensitive properties and encoded image information with cellular deformations triggered by light stimulation. The photoresponsive properties of the cells were determined using a single pixel imaging system, which indicated that the cells can act as a good light-sensitive biosensor. Additionally, the imaging feasibility of the cells was further validated through successful and clear imaging of several object scenes using the same system. This work represents a step toward the design and use of living cells as an image biosensor for the development of a new generation of high-resolution visual prostheses.
资助项目	National Key R & D Program of China[2018YFB1304700] ; Natural Science Foundation of Jiangsu Province of China[BK20180592] ; National Natural Science Foundation of China[91748212] ; National Natural Science Foundation of China[U1613220] ; National Natural Science Foundation of China[U1813210] ; National Natural Science Foundation of China[91848201] ; National Natural Science Foundation of China[61573341] ; National Natural Science Foundation of China[61727811] ; Key Research Program of Frontier Sciences, CAS[QYZDB-SSW-JSC008]
WOS关键词	RETINAL PROSTHESES ; IMPLANTATION ; TRIAL
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
WOS记录号	WOS:000476564300046
资助机构	National Key R & D Program of China ; Natural Science Foundation of Jiangsu Province of China ; National Natural Science Foundation of China ; Key Research Program of Frontier Sciences, CAS
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/25317]
专题	沈阳自动化研究所_机器人学研究室
通讯作者	Liu LQ(刘连庆); Wang WX(王文学)
作者单位	1.University of Chinese Academy of Sciences, Beijing 100049, China 2.School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China 3.Department of Chemistry, Stanford University, Stanford, CA 94305, United States 4.Key Laboratory of Advanced Process Control for Light Industry (Ministry of Education), Institute of Automation, Jiangnan University, Wuxi 214122, China 5.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China 6.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
推荐引用方式 GB/T 7714	Yang WG,Li GX,Wang FF,et al. Development of an image biosensor based on an optogenetically engineered cell for visual prostheses[J]. Nanoscale,2019,11(28):13213-13218.
APA	Yang WG.,Li GX.,Wang FF.,Liu LQ.,Wang WX.,...&Yang J.(2019).Development of an image biosensor based on an optogenetically engineered cell for visual prostheses.Nanoscale,11(28),13213-13218.
MLA	Yang WG,et al."Development of an image biosensor based on an optogenetically engineered cell for visual prostheses".Nanoscale 11.28(2019):13213-13218.