Assessment of mouse BMMNC DNA damage with a two-tailed comet assay after X-ray and carbon ion total body irradiation

doi:10.1007/s41365-018-0421-1

	Assessment of mouse BMMNC DNA damage with a two-tailed comet assay after X-ray and carbon ion total body irradiation
	Wang, Zhuan-Zi1 ; Li, Wen-Jian1 ; Wei, Wei1 ; Dang, Bing-Rong1 ; Liu, Fang 1,2
刊名	NUCLEAR SCIENCE AND TECHNIQUES
	2018-06
卷号	29 页码:77
关键词	Two-tailed comet assay, carbon ion irradiation X-ray irradiation Bone marrow mononuclear cells DNA damage
ISSN号	1001-8042
DOI	10.1007/s41365-018-0421-1
英文摘要	The two-tailed comet assay (2T-comet assay) is a method for simultaneously evaluating DNA single-strand breaks (SSBs) and double-strand breaks (DSBs). In the present study, the endonuclease DNase I and hydrogen peroxide were used to induce DSBs and SSBs in bone marrow mononuclear cells (BMMNCs) from mice, and the damaged DNAs were assessed with a 2T-comet assay. The results demonstrated that this method can detect and discriminate between BMMNC DNA SSBs and DSBs simultaneously. Using this method, we studied DNA damage in BMMNCs from female BALB/c mice after total body irradiation with X-rays and carbon ions. The results indicated that these two types of radiation induced serious DNA damage in BMMNCs in a dose-dependent manner. The DNA damage induced by carbon ions was more severe than that induced by X-rays at the same dose, and a high dose of carbon ion radiation was more likely to cause death in mice. The DSBs and SSBs induced by X-rays were the highest on the 3rd day post-IR. For carbon ion radiation, DSBs were the most serious on the 3rd day, while SSBs were more obvious on the 3rd day and 13th day post-IR. The ratio of DSBs/SSBs was clearly related to the different types of radiation.
资助项目	National Natural Science Foundation of China[11575259]
WOS关键词	DOUBLE-STRAND BREAKS ; OXIDATIVE STRESS ; BONE-MARROW ; IONIZING-RADIATION ; FE-56 IONS ; STEM-CELLS ; REPAIR ; INJURY ; MICE ; SENESCENCE
WOS研究方向	Nuclear Science & Technology ; Physics
语种	英语
出版者	SPRINGER SINGAPORE PTE LTD
WOS记录号	WOS:000433263900001
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/56833]
专题	近代物理研究所_生物物理研究室
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Zhuan-Zi,Li, Wen-Jian,Wei, Wei,et al. Assessment of mouse BMMNC DNA damage with a two-tailed comet assay after X-ray and carbon ion total body irradiation[J]. NUCLEAR SCIENCE AND TECHNIQUES,2018,29:77.
APA	Wang, Zhuan-Zi,Li, Wen-Jian,Wei, Wei,Dang, Bing-Rong,&Liu, Fang.(2018).Assessment of mouse BMMNC DNA damage with a two-tailed comet assay after X-ray and carbon ion total body irradiation.NUCLEAR SCIENCE AND TECHNIQUES,29,77.
MLA	Wang, Zhuan-Zi,et al."Assessment of mouse BMMNC DNA damage with a two-tailed comet assay after X-ray and carbon ion total body irradiation".NUCLEAR SCIENCE AND TECHNIQUES 29(2018):77.