Induction of reproductive cell death in Caenorhabditis elegans across entire linear-energy-transfer range of carbon-ion irradiation

doi:10.1016/j.dnarep.2018.01.009

	Induction of reproductive cell death in Caenorhabditis elegans across entire linear-energy-transfer range of carbon-ion irradiation
	Wu, Lijun 3; Chen, Liangwen 2,3; Tang, Huangqi 2,3; Du, Yan1 ; Dai, Zhangyu 2,3; Wang, Ting 3; Zhou, Libin 1; Bian, Po 3
刊名	DNA REPAIR
	2018-03-01
卷号	63 页码:39-46
关键词	Carbon-ion irradiation Bragg peak Range-distribution Reproductive cell death DNA damage response Vulval tissue model
ISSN号	1568-7864
DOI	10.1016/j.dnarep.2018.01.009
英文摘要	Heavy-ion radiation has attracted extensive attention as an effective cancer therapy because of the varying energy deposition along its track and its high cell-killing effect. Reproductive cell death (RCD), also known as clonogenic death, is an important mode of death of the cancer cells after radiotherapy. Although RCD induced by heavy-ion irradiation with various linear energy transfers has been demonstrated using clonogenic assay in vitro, little is known about the distribution of RCD across the range of heavy-ion irradiation at the level of whole organisms. In this study, a vulval tissue model of Caenorhabditis elegans was for the first time used to assess RCD in vivo induced by carbon-ion irradiation. A polymethyl methacrylate wedge was designed to provide a gradually varying thickness of shielding, so worms could be exposed to the entire range of carbon-ion irradiation. The carbon-ion irradiation led to a significant induction of RCD over the entire range in a dose-dependent manner. The biological peak did not correspond to the physical Bragg peak and moved forward, rather than spread forward, as radiation dose increased. The degree and shape of the range-distribution of RCD were also affected by the developmental stages of the worms. The gene mutations in DNA-damage checkpoints did not affect the responses of mutant worms positioned in biological peaks, compared to wild-type worms, but decreased radio sensitivity in the entrance region. An increased induction of RCD was observed in the worms impaired in homologous recombination (HR), but not in non-homologous end jointing pathway, suggesting a crucial role of HR repair in vulval cells of C. elegans in dealing with the carbon-ion-induced DNA damage. These unique manifestations of RCD in vivo in response to carbon-ion irradiation might provide new clues for further investigating the biological effects of heavy-ion irradiation.
资助项目	National Natural Science Foundation of China[U1332127] ; National Natural Science Foundation of China[11575233] ; National Natural Science Foundation of China[U1732144] ; Youth Innovation Promotion Association of Chinese Academy of Sciences[2017485]
WOS关键词	CANCER STEM-CELLS ; HUMAN FIBROBLASTS ; DNA-DAMAGE ; RADIOADAPTIVE RESPONSE ; TISSUE MODEL ; BRAGG CURVE ; HEAVY-IONS ; IN-VITRO ; X-RAYS ; RADIATION
WOS研究方向	Genetics & Heredity ; Toxicology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000427341600005
资助机构	National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/46360]
专题	近代物理研究所_生物物理研究室
通讯作者	Zhou, Libin; Bian, Po
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Biophys Grp, Lanzhou 730000, Gansu, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Wu, Lijun,Chen, Liangwen,Tang, Huangqi,et al. Induction of reproductive cell death in Caenorhabditis elegans across entire linear-energy-transfer range of carbon-ion irradiation[J]. DNA REPAIR,2018,63:39-46.
APA	Wu, Lijun.,Chen, Liangwen.,Tang, Huangqi.,Du, Yan.,Dai, Zhangyu.,...&Bian, Po.(2018).Induction of reproductive cell death in Caenorhabditis elegans across entire linear-energy-transfer range of carbon-ion irradiation.DNA REPAIR,63,39-46.
MLA	Wu, Lijun,et al."Induction of reproductive cell death in Caenorhabditis elegans across entire linear-energy-transfer range of carbon-ion irradiation".DNA REPAIR 63(2018):39-46.