Influence of the environment and phototoxicity of the live cell imaging system at IMP microbeam facility

doi:10.1016/j.nimb.2017.01.076

	Influence of the environment and phototoxicity of the live cell imaging system at IMP microbeam facility
	Du, Guanghua3 ; Liu, Wenjing 3; Wu, Ruqun 2,3; Wei, Junzhe 2,3; Chen, Hao 1; Li, Xiaoyue 2,3; Li, Yaning 2,3; Zhao, Jing 2,3; Guo, Jinlong3,4
	2017-08
关键词	Live cell imaging DNA damage and repair Phototoxicity Environment control Microbeam
卷号	404
DOI	10.1016/j.nimb.2017.01.076
页码	125-130
英文摘要	To investigate the spatiotemporal dynamics of DNA damage and repair after the ion irradiation, an online live cell imaging system has been established based on the microbeam facility at Institute of Modern Physics (IMP). The system could provide a sterile and physiological environment by making use of heating plate and live cell imaging solution. The phototoxicity was investigated through the evaluation of DNA repair protein XRCC1 foci formed in HT1080-RFP cells during the imaging exposure. The intensity of the foci induced by phototoxicity was much lower compared with that of the foci induced by heavy ion hits. The results showed that although spontaneous foci were formed due to RFP exposure during live cell imaging, they had little impact on the analysis of the recruitment kinetics of XRCC1 in the foci induced by the ion irradiation. (C) 2017 Elsevier B.V. All rights reserved.
会议录	NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
会议录出版者	ELSEVIER SCIENCE BV
会议录出版地	PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
语种	英语
资助项目	Ministry of Science and Technology[2012YQ03014204]
WOS研究方向	Instruments & Instrumentation ; Nuclear Science & Technology ; Physics
WOS记录号	WOS:000404709900024
内容类型	会议论文
源URL	[http://119.78.100.186/handle/113462/58158]
专题	近代物理研究所_实验物理中心
通讯作者	Du, Guanghua
作者单位	1.Univ Lanzhou, Lanzhou, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China 4.Northwest Normal Univ, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Du, Guanghua,Liu, Wenjing,Wu, Ruqun,et al. Influence of the environment and phototoxicity of the live cell imaging system at IMP microbeam facility[C]. 见:.