Anisotropic aspects of solubility behavior in the demineralization of cortical bone revealed by XRD analysis

doi:10.1007/s10867-018-9516-5

	Anisotropic aspects of solubility behavior in the demineralization of cortical bone revealed by XRD analysis
	Danilchenko, Sergei 1; Kalinkevich, Aleksei 1; Zhovner, Mykhailo 1; Kuznetsov, Vladimir 1; Li, He2 ; Wang, Jufang2
刊名	JOURNAL OF BIOLOGICAL PHYSICS
	2019-03-01
卷号	45 期号:1 页码:77-88
关键词	Apatite X-ray diffraction Cortical bone Crystal orientation Morphology Demineralization Crystallite size
ISSN号	0092-0606
DOI	10.1007/s10867-018-9516-5
通讯作者	Kalinkevich, Aleksei(kalinkevich@gmail.com)
英文摘要	Dissolution of cortical bone mineral under demineralization in 0.1M HCl and 0.1M EDTA solutions is studied by X-ray diffraction (XRD). The bone specimens (in the form of planar oriented pieces) were cut from a diaphysial fragment of a mature mammal bone so that a cross-section surface and a longitudinal section surface could be analyzed individually. This permitted to compare the dissolution behavior of bone apatite of different morphologies: crystals having the c-axis of the hexagonal unit-cell generally parallel to the long axis of the bone (major morphology) and those having the c-axis almost perpendicular to the bone axis (minor morphology). For these two types of morphology, the crystallite sizes in two mutually perpendicular directions (namely, [002] and [310]) were estimated by Scherrer formula in the initial and the stepwise-demineralized specimens. The data obtained reveal that the crystals belonging to the minor morphology dissolve faster than the crystals of the major morphological type, despite the fact that the crystallites of the minor morphology seem to be only a little smaller than those of the major morphology; the apatite crystallites irrespective of the morphology type are elongated in the c-axis direction. We hypothesize that the revealed difference in solubility may be caused by diverse chemical modifications of apatite of these two morphological types, since the solubility of apatite is strictly regulated by anionic and cationic substitutions in the lattice. The anisotropy effect in solubility of bone mineral seems to be functionally predetermined and this should be a crucial factor in the resorption and remodeling behavior of a bone. Some challenges arising at XRD examination of partially decalcified cortical bone blocks are discussed, as well as the limitations of estimation of bone crystallite size by XRD line-broadening analysis.
资助项目	International Science & Technology Cooperation Program of China (ISTCP)[2015DFR30940] ; Chinese Academy of Science ; State Administration of Foreign Experts Affairs P.R. China
WOS关键词	X-RAY-DIFFRACTION ; CRYSTALLITE SIZE ; CARBONATED APATITE ; MODEL ; ORIENTATION ; SHAPE
WOS研究方向	Biophysics
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000460742900006
资助机构	International Science & Technology Cooperation Program of China (ISTCP) ; Chinese Academy of Science ; State Administration of Foreign Experts Affairs P.R. China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/134389]
专题	中国科学院近代物理研究所
通讯作者	Kalinkevich, Aleksei
作者单位	1.NAS Ukraine, Inst Appl Phys, Sumy, Ukraine 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Danilchenko, Sergei,Kalinkevich, Aleksei,Zhovner, Mykhailo,et al. Anisotropic aspects of solubility behavior in the demineralization of cortical bone revealed by XRD analysis[J]. JOURNAL OF BIOLOGICAL PHYSICS,2019,45(1):77-88.
APA	Danilchenko, Sergei,Kalinkevich, Aleksei,Zhovner, Mykhailo,Kuznetsov, Vladimir,Li, He,&Wang, Jufang.(2019).Anisotropic aspects of solubility behavior in the demineralization of cortical bone revealed by XRD analysis.JOURNAL OF BIOLOGICAL PHYSICS,45(1),77-88.
MLA	Danilchenko, Sergei,et al."Anisotropic aspects of solubility behavior in the demineralization of cortical bone revealed by XRD analysis".JOURNAL OF BIOLOGICAL PHYSICS 45.1(2019):77-88.