Epithelial-mesenchymal transition in non-targeted lung tissues of Kunming mice exposed to X-rays is suppressed by celecoxib

doi:10.1093/jrr/rry050

	Epithelial-mesenchymal transition in non-targeted lung tissues of Kunming mice exposed to X-rays is suppressed by celecoxib
	Hu, Wentao 1,2; Pei, Hailong 1,2; Sun, Fang 3; Li, Pengfei 3; Nie, Jing 1,2; Li, Bingyan 4; Hei, Tom K.5; Zhou, Guangming1,2
刊名	JOURNAL OF RADIATION RESEARCH
	2018-09
卷号	59 页码:583-587
关键词	radiation-induced non-targeted effect mouse epithelial-mesenchymal transition cyclooxygenase-2
ISSN号	0449-3060
DOI	10.1093/jrr/rry050
英文摘要	Lung cancer is one of the highest health risks caused by ionizing radiation, which induces both direct effects and non-targeted effects. However, whether radiation-induced non-targeted effects result in epithelial-mesenchymal transition, a critical process during tumorigenesis, in non-targeted lung tissues remains unknown. In the present study, Kunming mice were subjected to whole-body, cranial or local abdominal irradiation of single-dose or fractionated 4 Gy X-rays, and the expressions of epithelial-mesenchymal transition markers in non-targeted lung tissues were assessed by both qRT-PCR and immunofluorescent staining. It was found that the epithelial marker was downregulated while the mesenchymal markers were upregulated significantly in non-targeted lung tissues of the irradiated mice. Local abdominal irradiation was more efficient in inducing epithelial-mesenchymal transition than whole-body or cranial irradiation when the fractionated irradiation method was adopted. In addition, the intraperitoneal administration of celecoxib suppressed epithelial-mesenchymal transition in the non-targeted lung tissues. In conclusion, our findings suggest that epithelial-mesenchymal transition is induced in non-targeted lung tissues, but can be suppressed by inhibition of cyclooxygenase-2 by celecoxib.
资助项目	National Natural Science Foundation of China[11405235] ; National Natural Science Foundation of China[11335011]
WOS关键词	CANCER ; RADIATION ; BYSTANDER ; RADIOTHERAPY ; EXPRESSION ; CELLS ; MODEL
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Oncology ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000452744800007
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/64477]
专题	中国科学院近代物理研究所
通讯作者	Zhou, Guangming
作者单位	1.Soochow Univ, Sch Radiat Med & Protect, Med Coll, 199 Renai Rd, Suzhou 215123, Peoples R China 2.Collaborat Innovat Ctr Radiol Med Jiangsu Higher, Suzhou 215123, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Gansu Key Lab Space Radiobiol, Lanzhou 730000, Gansu, Peoples R China 4.Soochow Univ, Med Coll, Suzhou 215123, Peoples R China 5.Columbia Univ, Coll Phys & Surg, Ctr Radiol Res, New York, NY 10032 USA
推荐引用方式 GB/T 7714	Hu, Wentao,Pei, Hailong,Sun, Fang,et al. Epithelial-mesenchymal transition in non-targeted lung tissues of Kunming mice exposed to X-rays is suppressed by celecoxib[J]. JOURNAL OF RADIATION RESEARCH,2018,59:583-587.
APA	Hu, Wentao.,Pei, Hailong.,Sun, Fang.,Li, Pengfei.,Nie, Jing.,...&Zhou, Guangming.(2018).Epithelial-mesenchymal transition in non-targeted lung tissues of Kunming mice exposed to X-rays is suppressed by celecoxib.JOURNAL OF RADIATION RESEARCH,59,583-587.
MLA	Hu, Wentao,et al."Epithelial-mesenchymal transition in non-targeted lung tissues of Kunming mice exposed to X-rays is suppressed by celecoxib".JOURNAL OF RADIATION RESEARCH 59(2018):583-587.