Experimental research of radiogenic therapy on human melanoma

	Experimental research of radiogenic therapy on human melanoma
	Min, Fengling ; Zhang, Hong; Li, Wenjiang ; Gao, Qingxiang ; Liu, Bing ; Zhou, Qingming ; Duan, Xin ; Zhou, Guangming
刊名	He Jishu/Nuclear Techniques
	2006
卷号	29 页码:442-447
ISSN号	0253-3219
英文摘要	To investigate the effect of low dose irradiation on gene transfer efficiency and the effect of adenoviral-mediated exogenous P53 overexpression on radiosensitivity of radioresistant human melanoma cell line A375 with wild type p53, control vector, a replication deficient recombinant adenoviral vector containing a CMV promoter and green fluorescent protein (AdCMV-GFP), was used to transfect the A375 cells preirradiated with or without 1 Gy X-ray radiation. The transduction efficiency of GFP gene was determined with fluorescence microscope directly. A375 cells radiated by 1 Gy X-ray were transfected with a replication deficient recombinant adenoviral vector carrying human wild p53 (AdCMV-P53), and mRAN level was detected by RT-PCR. The cell cycle delay and the expression of exogenous P53 were detected using flow cytometry (FCM) at different time after transfection. The radiosensitivity of A375 cells after p53 transduction was assayed by clonoy formation. We found that IGy exposure increased the gene transfer efficiency of A375 cells. The expression of exogenous P53 was found to be 60% to 80% of transfected cells during the first three days after transduction and then declined continuously down to the control level on the day 10. The G1 cell cycle arrest was also observed after p53 gene transfer. A375 cells that were transfected with p53 showed higher sensitivity to X-ray induced cell killing than those cells that either were transfected with the viral vector carrying a green fluorescent protein gene or were not transfected at all. Low dose ionizing radiation can improve gene transfer efficiency of A375 cells mediated by adenovirus vector. Although the overexpression of exogenous P53 may not inhibit cell growth and induce apoptosis of melanoma cell line A375 in vitro, it made the tumor cells much sensitive to death by irradiation. The data suggested that p53 gene might be a potential gene for melanoma therapy and provide the experimental evidences to clinically using the combination of radiation with gene therapy on melanoma. Namely, there may be a reduction of side effects resulting from high dose radiation on radioresistant tumors or rAd-P53 administration alone in clinical applications.
出版者	Science Press, Beijing, China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/64175]
专题	中国科学院近代物理研究所
作者单位	1.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China 2.Graduate School, Chinese Academy of Sciences, Beijing 100049, China 3.School of Life Science, Lanzhou University, Lanzhou 730000, China
推荐引用方式 GB/T 7714	Min, Fengling,Zhang, Hong,Li, Wenjiang,et al. Experimental research of radiogenic therapy on human melanoma[J]. He Jishu/Nuclear Techniques,2006,29:442-447.
APA	Min, Fengling.,Zhang, Hong.,Li, Wenjiang.,Gao, Qingxiang.,Liu, Bing.,...&Zhou, Guangming.(2006).Experimental research of radiogenic therapy on human melanoma.He Jishu/Nuclear Techniques,29,442-447.
MLA	Min, Fengling,et al."Experimental research of radiogenic therapy on human melanoma".He Jishu/Nuclear Techniques 29(2006):442-447.