Inhibition of human colorectal adenocarcinoma cells with AdCMV-p53 gene transfection induced by irradiation

	Inhibition of human colorectal adenocarcinoma cells with AdCMV-p53 gene transfection induced by irradiation
	Liu, Bing ; Zhang, Hong; Li, Wenjian; Gao, Qingxiang ; Min, Fengling ; Xie, Yi; Zhou, Qingming ; Duan, Xin ; Zhou, Guangming
刊名	He Jishu/Nuclear Techniques
	2006
卷号	29 页码:674-678
ISSN号	0253-3219
英文摘要	The effect of AdCMV-p53 gene transfection induced by y-ray irradiation on human colorectal adenocarcinoma cells was investigated. The HT-29 cells were irradiated by 0.5, 1.0, 2.0 Gy ⁶⁰Co y-rays, then were transfected with AdCMV-GFP (a replication of deficient recombinant adenoviral vector containing a CMV promoter and green fluorescent protein) or AdCMV-p53 (a replication of deficient recombinant adenoviral vector containing a CMV promoter and carrying human wild p53 gene). Cytotoxity was measured by clonogenic survival assay; apoptosis and the p53 expression were determined by flow cytometry. The results show that the pre-exposure of 0.5 Gy ⁶⁰Co y-rays significantly enhanced the inhibition of HT-29 cells with AdCMV-p53 transfection and promoted cell apoptosis. The inhibition rates for the groups of pre-exposure with 0.5 Gy and transfection with 40 and 80 MOI AdCMV-p53 were 50% and 20% higher than those for the groups of the mere transfection, and 40% more than the mere irradiation group. In the case of higher than 0.5 Gy pre-exposure, no significant difference was found between the pre-exposure with transfection group and the mere irradiation group. So 0.5 Gy pre-irradiation and AdCMV-p53 transfection obviously increases the inhibition of HT-29 cells with AdCMV-p53 transfection. The optimum condition is the lower than 1.0 Gy pre-exposure combined with the lower than 80 MOI AdCMV-p53 transfection.
出版者	Science Press, Beijing, China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/64140]
专题	中国科学院近代物理研究所
作者单位	1.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China 2.Graduate School, Chinese Academy of Sciences, Beijing 100049, China 3.School of Life Science, Lanzhou University, Lanzhou 730000, China
推荐引用方式 GB/T 7714	Liu, Bing,Zhang, Hong,Li, Wenjian,et al. Inhibition of human colorectal adenocarcinoma cells with AdCMV-p53 gene transfection induced by irradiation[J]. He Jishu/Nuclear Techniques,2006,29:674-678.
APA	Liu, Bing.,Zhang, Hong.,Li, Wenjian.,Gao, Qingxiang.,Min, Fengling.,...&Zhou, Guangming.(2006).Inhibition of human colorectal adenocarcinoma cells with AdCMV-p53 gene transfection induced by irradiation.He Jishu/Nuclear Techniques,29,674-678.
MLA	Liu, Bing,et al."Inhibition of human colorectal adenocarcinoma cells with AdCMV-p53 gene transfection induced by irradiation".He Jishu/Nuclear Techniques 29(2006):674-678.