A Combined Array-Based Comparative Genomic Hybridization and Functional Library Screening Approach Identifies mir-30d As an Oncomir in Cancer

doi:10.1158/0008-5472.CAN-11-2484

	A Combined Array-Based Comparative Genomic Hybridization and Functional Library Screening Approach Identifies mir-30d As an Oncomir in Cancer
	Li, Ning1,2,7 ; Kaur, Sippy 5,6; Greshock, Joel 3; Lassus, Heini 5,6; Zhong, Xiaomin 1,2; Wang, Yanling 1,2; Leminen, Arto 5,6; Shao, Zhongjun 1,2; Hu, Xiaowen 1,2; Liang, Shun 1,2
刊名	CANCER RESEARCH
	2012
卷号	72 页码:154-164
ISSN号	0008-5472
DOI	10.1158/0008-5472.CAN-11-2484
文献子类	Article
英文摘要	Oncomirs are microRNAs (miRNA) that acts as oncogenes or tumor suppressor genes. Efficient identification of oncomirs remains a challenge. Here we report a novel, clinically guided genetic screening approach for the identification of oncomirs, identifying mir-30d through this strategy. mir-30d regulates tumor cell proliferation, apoptosis, senescence, and migration. The chromosomal locus harboring mir-30d was amplified in more than 30% of multiple types of human solid tumors (n=1,283). Importantly, higher levels of mir-30d expression were associated significantly with poor clinical outcomes in ovarian cancer patients (n-330, P-0.0016). Mechanistic investigations suggested that mir-30d regulates a large number of cancer-associated genes, including the apoptotic caspase CASP3. The guided genetic screening approach validated by this study offers a powerful tool to identify oncomirs that may have utility as biomarkers or targets for drug development. Cancer Res; 72(1); 154-64. (C) 2011 AACR.
资助项目	Ovarian Cancer SPORE[P50-CA83638-7951]
WOS关键词	CHRONIC LYMPHOCYTIC-LEUKEMIA ; MICRORNA SIGNATURES ; TUMOR-SUPPRESSOR ; GENE-EXPRESSION ; LUNG-CANCER ; C-ELEGANS ; RNAS ; METASTASIS ; CELLS ; INVASION
WOS研究方向	Oncology
语种	英语
出版者	AMER ASSOC CANCER RESEARCH
WOS记录号	WOS:000298755600016
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/48599]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Lin
作者单位	1.Univ Penn, Ovarian Canc Res Ctr, Philadelphia, PA 19104 USA 2.Univ Penn, Dept Obstet & Gynecol, Philadelphia, PA 19104 USA 3.Univ Penn, Abramson Family Canc Res Inst, Philadelphia, PA 19104 USA 4.Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA 5.Univ Helsinki, Dept Obstet & Gynecol & Pathol, FIN-00014 Helsinki, Finland 6.Helsinki Univ Cent Hosp, Helsinki, Finland 7.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China 8.Univ Turin, Dept Obstet & Gynecol, Turin, Italy
推荐引用方式 GB/T 7714	Li, Ning,Kaur, Sippy,Greshock, Joel,et al. A Combined Array-Based Comparative Genomic Hybridization and Functional Library Screening Approach Identifies mir-30d As an Oncomir in Cancer[J]. CANCER RESEARCH,2012,72:154-164.
APA	Li, Ning.,Kaur, Sippy.,Greshock, Joel.,Lassus, Heini.,Zhong, Xiaomin.,...&Zhang, Lin.(2012).A Combined Array-Based Comparative Genomic Hybridization and Functional Library Screening Approach Identifies mir-30d As an Oncomir in Cancer.CANCER RESEARCH,72,154-164.
MLA	Li, Ning,et al."A Combined Array-Based Comparative Genomic Hybridization and Functional Library Screening Approach Identifies mir-30d As an Oncomir in Cancer".CANCER RESEARCH 72(2012):154-164.