Serum microRNAs as Early Indicators for Estimation of Exposure Degree in Response to Ionizing Irradiation

doi:10.1667/RR14702.1

	Serum microRNAs as Early Indicators for Estimation of Exposure Degree in Response to Ionizing Irradiation
	Wei, Wenjun 1,2,3; He, Jinpeng1,2 ; Wang, Jufang 1,2,4; Ding, Nan 1,2; Wang, Bing 1,2,3; Lin, Sulan 1,2,3; Zhang, Xurui 1,2,3; Hua, Junrui 1,2; Li, He 1,2,3; Hu, Burong 1,2,4
刊名	RADIATION RESEARCH
	2017-09-01
卷号	188 页码:342-354
ISSN号	0033-7587
DOI	10.1667/RR14702.1
英文摘要	Exposure to ionizing radiation from nuclear devices, spaceflights or terrorist attacks represents a major threat to human health and public security. After a radiological incident, noninvasive biomarkers that can facilitate rapid assessment of exposure risk in the early stages are urgently needed for optimal medical treatment. Serum microRNAs (miRNAs) are ideal biomarkers because they are stable in response to environmental changes, they are common among different species and are easily collected. Here, we performed miRNA PCR arrays to analyze miRNA expression profiles at 24 h postirradiation. Blood samples were collected from animals that received 0.5-2 Gy total-body carbon-ion irradiation. A specific signature with 12 radiosensitive miRNAs was selected for further validation. After exposure to 0.1-2 Gy of carbon-ion, iron-ion or X-ray radiations, five miRNAs that showed a significant response to these radiation types were selected for further observation of dose-and timedependent changes: miR-183-5p, miR-9-3p, miR-200b-5p, miR-342-3p and miR-574-5p. We developed a universal model using these five miRNAs to predict the degree of exposure to different radiation types with high sensitivity and specificity. In conclusion, we have identified a set of miRNAs that are quite sensitive to different radiation types in the early stages after exposure, demonstrating their potential use as effective indicators to predict the degree of exposure. (C) 2017 by Radiation Research Society
资助项目	Concept Research of Space Science Mission of Chinese Academy of Sciences[XDA15007803] ; International Science and Technology Cooperation Program of China[2015DFR30940] ; National Natural Sciences Foundation of China[31270895] ; National Natural Sciences Foundation of China[11405232] ; National Natural Sciences Foundation of China[31400723]
WOS关键词	CELL LUNG-CANCER ; HEPATOCELLULAR-CARCINOMA ; CIRCULATING MICRORNA ; BIOMARKER ; CONSERVATION ; BIODOSIMETRY ; EXPRESSION ; IDENTIFICATION ; LYMPHOCYTES ; METASTASIS
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Biophysics ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	RADIATION RESEARCH SOC
WOS记录号	WOS:000408424300009
资助机构	Concept Research of Space Science Mission of Chinese Academy of Sciences ; International Science and Technology Cooperation Program of China ; National Natural Sciences Foundation of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/45588]
专题	中国科学院近代物理研究所
通讯作者	Wang, Jufang
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Collaborat Innovat Ctr Radiat Med Jiangsu Higher, Suzhou, Peoples R China
推荐引用方式 GB/T 7714	Wei, Wenjun,He, Jinpeng,Wang, Jufang,et al. Serum microRNAs as Early Indicators for Estimation of Exposure Degree in Response to Ionizing Irradiation[J]. RADIATION RESEARCH,2017,188:342-354.
APA	Wei, Wenjun.,He, Jinpeng.,Wang, Jufang.,Ding, Nan.,Wang, Bing.,...&Hu, Burong.(2017).Serum microRNAs as Early Indicators for Estimation of Exposure Degree in Response to Ionizing Irradiation.RADIATION RESEARCH,188,342-354.
MLA	Wei, Wenjun,et al."Serum microRNAs as Early Indicators for Estimation of Exposure Degree in Response to Ionizing Irradiation".RADIATION RESEARCH 188(2017):342-354.