Xanthohumol induces paraptosis of leukemia cells through p38 mitogen activated protein kinase signaling pathway

doi:10.18632/oncotarget.16185

	Xanthohumol induces paraptosis of leukemia cells through p38 mitogen activated protein kinase signaling pathway
	Mi, Xiangquan 1,2; Wang, Chunming 1; Sun, Chao 3; Chen, Xu 1; Huo, Xiang 1; Zhang, Yiming 2; Li, Gang 2; Xu, Bo 2; Zhang, Jun 4; Xie, Jianxin 4
刊名	ONCOTARGET
	2017-05-09
卷号	8 页码:31297-31304
关键词	xanthohumol paraptosis p38-MAPK cytoplasmic vacuolation ER stress
ISSN号	1949-2553
DOI	10.18632/oncotarget.16185
英文摘要	Xanthohumol as a natural polyphenol demonstrates an anticancer activity, but its underlying mechanism remains unclear. In this study, we showed that xanthohumol (XN) induces paraptosis of leukemia cells. The paraptosis is one cell death which is characterized by dilation of the endoplasmic reticulum and/or mitochondria. The results demonstrated that XN treatment significantly inhibited cell proliferation and triggered extensive cytoplasmic vacuolation of HL-60 leukemia cells, but it did not cause the cleavage of caspase-3 protein or apoptosis. In contrast, XN treatment resulted in LC3-II accumulation through blocking of autophagosome maturation. Interestingly, the induction of cytoplasmic vacuolization by XN is not associated with autophagy modulated by XN, therefore, XN-induced cell death of HL-60 leukemia cells is not the classical apoptotic cell death. Intriguingly, XN treatment triggered the dilatation of endoplasma reticulum (ER) and induced ER stress by upregulating C/EBP homologous protein and unfolded protein response regulator Grp78/Bip. Furthermore, XN treatment triggered p38 mitogen activated protein kinase and its specific inhibitor inhibited the paraptosis of HL-60 leukemia cells by XN. In conclusion, we for the first time demonstrated that XN treatment can induce paraptosis of leukemia cells through activation of p38 MAPK signaling.
资助项目	National Natural Science Foundation of China[21372190] ; National Natural Science Foundation of China[31470426] ; Taishan Scholar Program of Shandong Province[tshw201502046] ; Yantai ShuangBai Scholar Program
WOS关键词	CYTOPLASMIC VACUOLATION DEATH ; CANCER-CELLS ; HUMULUS-LUPULUS ; APOPTOSIS ; INDUCTION ; AUTOPHAGY ; STRESS
WOS研究方向	Oncology ; Cell Biology
语种	英语
出版者	IMPACT JOURNALS LLC
WOS记录号	WOS:000401003200054
资助机构	National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; Yantai ShuangBai Scholar Program
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/44746]
专题	中国科学院近代物理研究所
通讯作者	Li, Ji
作者单位	1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Gansu, Peoples R China 2.Yantai Univ, Coll Life Sci, Ctr Mitochondrial & Hlth Aging, Yantai 264005, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 4.Shihezi Univ, Sch Med, Shihezi 832000, Xinjiang, Peoples R China
推荐引用方式 GB/T 7714	Mi, Xiangquan,Wang, Chunming,Sun, Chao,et al. Xanthohumol induces paraptosis of leukemia cells through p38 mitogen activated protein kinase signaling pathway[J]. ONCOTARGET,2017,8:31297-31304.
APA	Mi, Xiangquan.,Wang, Chunming.,Sun, Chao.,Chen, Xu.,Huo, Xiang.,...&Li, Ji.(2017).Xanthohumol induces paraptosis of leukemia cells through p38 mitogen activated protein kinase signaling pathway.ONCOTARGET,8,31297-31304.
MLA	Mi, Xiangquan,et al."Xanthohumol induces paraptosis of leukemia cells through p38 mitogen activated protein kinase signaling pathway".ONCOTARGET 8(2017):31297-31304.