RAC2-P38 MAPK-dependent NADPH oxidase activity is associated with the resistance of quiescent cells to ionizing radiation

doi:10.1080/15384101.2016.1259039

	RAC2-P38 MAPK-dependent NADPH oxidase activity is associated with the resistance of quiescent cells to ionizing radiation
	Pei, Hailong 1,2,6; Zhang, Jian 1,2,6; Nie, Jing 1,2,6; Ding, Nan3 ; Hu, Wentao 1,2,6; Hua, Junrui3 ; Hirayama, Ryoichi 4; Furusawa, Yoshiya 4; Liu, Cuihua 4; Li, Bingyan 1,2
刊名	CELL CYCLE
	2017
卷号	16 页码:113-122
关键词	NADPH oxidase quiescent cells RAC2 radiosensitivity reactive oxygen species
ISSN号	1538-4101
DOI	10.1080/15384101.2016.1259039
英文摘要	Our recent study showed that quiescent G0 cells are more resistant to ionizing radiation than G1 cells; however, the underlying mechanism for this increased radioresistance is unknown. Based on the relatively lower DNA damage induced in G0 cells, we hypothesize that these cells are exposed to less oxidative stress during exposure. As a catalytic subunit of NADPH oxidase, Ras-related C3 botulinum toxin substrate 2 (RAC2) may be involved in the cellular response to ionizing radiation. Here, we show that RAC2 was expressed at low levels in G0 cells but increased substantially in G1 cells. Relative to G1 cells, the total antioxidant capacity in G0 phase cells increased upon exposure to X-ray radiation, whereas the intracellular concentration of ROS and malondialdehyde increased only slightly. The induction of DNA single- and double-stranded breaks in G1 cells by X-ray radiation was inhibited by knockdown of RAC2. P38 MAPK interaction with RAC2 resulted in a decrease of functional RAC2. Increased phosphorylation of P38 MAPK in G0 cells also increased cellular radioresistance; however, excessive production of ROS caused P38 MAPK dephosphorylation. P38 MAPK, phosphorylated P38 MAPK, and RAC2 regulated in mutual feedback and negative feedback regulatory pathways, resulting in the radioresistance of G0 cells.
资助项目	National Natural Science Foundations of China[11335011] ; National Natural Science Foundations of China[31370846] ; National Natural Science Foundations of China[31400723] ; National Natural Science Foundations of China[11405235] ; National Natural Science Foundations of China[31600674] ; National Natural Science Foundations of China[81602794] ; China Postdoctoral Science Foundation[2016M591904] ; Natural Science Foundations of Jiangsu[BK20160334]
WOS关键词	GLUTATHIONE-REDUCTASE ; SIGNAL-TRANSDUCTION ; THIOREDOXIN SYSTEM ; FREE-RADICALS ; ROS ; GENERATION ; PATHWAY ; STRESS ; DNA ; MITOCHONDRIA
WOS研究方向	Cell Biology
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000392737300021
资助机构	National Natural Science Foundations of China ; China Postdoctoral Science Foundation ; Natural Science Foundations of Jiangsu
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/44247]
专题	中国科学院近代物理研究所
通讯作者	Zhou, Guangming
作者单位	1.Soochow Univ, Coll Med, Sch Radiat Med & Protect, Suzhou, Peoples R China 2.Collaborat Innovat Ctr Radiol Med Jiangsu Higher, Suzhou, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Dept Space Radiobiol, Lanzhou, Peoples R China 4.Natl Inst Quanturn & Radiol Sci & Technol, Res Ctr Charged Particle Therapy, Chiba, Japan 5.Columbia Univ Coll Phys & Surg, Ctr Radiol Res, 630 W 168th St, New York, NY 10032 USA 6.Soochow Univ, Inst Space Life Sci, Suzhou, Peoples R China
推荐引用方式 GB/T 7714	Pei, Hailong,Zhang, Jian,Nie, Jing,et al. RAC2-P38 MAPK-dependent NADPH oxidase activity is associated with the resistance of quiescent cells to ionizing radiation[J]. CELL CYCLE,2017,16:113-122.
APA	Pei, Hailong.,Zhang, Jian.,Nie, Jing.,Ding, Nan.,Hu, Wentao.,...&Zhou, Guangming.(2017).RAC2-P38 MAPK-dependent NADPH oxidase activity is associated with the resistance of quiescent cells to ionizing radiation.CELL CYCLE,16,113-122.
MLA	Pei, Hailong,et al."RAC2-P38 MAPK-dependent NADPH oxidase activity is associated with the resistance of quiescent cells to ionizing radiation".CELL CYCLE 16(2017):113-122.